鱼腥草组织培养中材料灭菌的研究  

Sterilization of Houttuynia cordata Thunbn Explant in Tissue Culture

作　　者：应明[1] 袁艺[1] 何斌[2] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]合肥市第一中学

出　　处：《安徽农学通报》2009年第4期27-29,共3页Anhui Agricultural Science Bulletin

基　　金：安徽省优秀青年基金;安徽省高校十五优秀人才基金资助。

摘　　要：采用不同的灭菌措施,包括不同的灭菌剂、浓度,对鱼腥草的带芽茎段进行灭菌处理,研究其污染率和污染速率的变化,寻找建立无菌培养系最适宜的方法。结果表明:最适宜的灭菌措施为:外植体茎+70%酒精浸30s+0.1%升汞10-12min+青霉素+链霉素15min。Houttuynia Cordata Thunb stems with leading bud were sterilized with different sterilizing meansures,：including different sterilizing pharmaceutical in different densities.The most suitable method for asepsis cultivation was set up by analysis the pollution rate and speed .Results were as follows： the most suitable sterilization method of explant was soaking them in 70% alcohol for 30s, putting them in 0.1% HgCL for 10mins or 12mins and soaking them in Penicillin,Streptomycin for 15mins.

关 键 词：鱼腥草 无菌培养系 污染率 

分 类 号：Q813.12[生物学—生物工程]