大豆抗疫霉根腐病的蛋白组研究  

Proteome Analysis on Resistance to Phytophora Root Rot in Soybean

作　　者：邱红梅[1,2] 刘春燕[1,3] 张代军[2] 辛秀君[2] 王家麟[3] 王晶[3] 单彩云[3] 单大鹏[3] 胡国华[1] 陈庆山[3] 

机构地区：[1]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090 [2]黑龙江省农垦总局红兴隆科研所,黑龙江友谊155811 [3]东北农业大学大豆研究所,黑龙江哈尔滨150030

出　　处：《作物学报》2009年第3期418-423,共6页Acta Agronomica Sinica

基　　金：引进国际先进农业科学技术计划(948计划)项目[2006-G1(A)];国家高技术研究发展计划(863计划)项目(2006AA10Z1F4);黑龙江省博士后科研启动基金项目(LHK-04014);黑龙江省博士后资助项目(LRB06-126);黑龙江省“十一五”科技攻关项目(GA06B101-2-6)资助

摘　　要：利用双向电泳及MALDI-TOF-MS技术分析绥农10号真叶接种疫霉菌1号生理小种后的蛋白质组变化。在抗病品种绥农10号叶片中共获得19个差异表达蛋白点,其中有12个上调表达,6个下调表达,1个特异表达(仅在接种后出现)。利用生物质谱分析8个上调表达点、1个下调表达点和1个特异表达点,最终鉴定得到8个有注释功能的蛋白,根据功能可分为4类,第1类为参与新陈代谢的蛋白,包括二磷酸核酮糖羧化酶的大亚基及前体、琥珀酰-辅酶A;第2类为参与信号传导的蛋白,包括激酶受体类蛋白、氧化还原酶和半胱氨酸氧化还原酶;第3类为参与细胞内物质运输的蛋白,包括衣壳蛋白的zeta-3亚基;第4类为转录因子,是参与茉莉酸介导的F-box蛋白。这些蛋白可为进一步研究大豆抗病机制奠定基础。Phytophthora root rot caused by Phytophthora sojae is a serious soil-borne fungi disease endangering soybean production and bringing huge economic losses. The objective of this study was to survey and identify the proteins associated with resistance, using soybean cultivar Suinong 10 by two-dimensional gel electrophoresis and mass spectrometry technology. Nineteen differentially expressed proteins were obtained, of twelve which presented up-regulated, six were down-regulated, and one was special protein. Ten protein spots including eight with up-regulated expression, one with down-regulated expression, and one with specially expression were investigated by biological mass spectrometry. The results showed that eight functional proteins were identified, three of which involved in metabolism, consisting of the ribolose-1,5-phosphate carboxylase large subunit and its precursor, succinyl coenzyme A, three of which were related to signal transduction, encompassing kinase receptor like protein, oxi- doreductase, and cysteamine ammonia acid oxidoreductase, one of which participated in transport of metabolites in cells, i.e. clothing zeta-3 protein subunits, one of which was concerned with the jasmonic acid-mediated resistance, including F-box protein. The results provide useful information for studying the molecular mechanism of resistance to Phytophthora root rot.

关 键 词：大豆 疫霉根腐病 抗病相关蛋白 2D MALDI-TOF-MS 

分 类 号：S565.1[农业科学—作物学]