猪Mighty基因的克隆与组织表达分析  

Cloning and Tissue Expression of Porcine Mighty Gene

作　　者：张志强[1] 李宏基[1] 王伟杰[1] 韩立强[1] 王静[1] 武宇晓[1] 臧猛[1] 台玉磊[1] 杨国宇[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002

出　　处：《江西农业学报》2009年第2期1-4,共4页Acta Agriculturae Jiangxi

基　　金：河南省重点科技攻关项目(编号:0523010500)

摘　　要：Mighty基因是在骨骼肌中发现的一个新颖的肌形成前期因子。基于电子延伸序列,本试验成功克隆出了Mighty基因,其全长874 bp,开放阅读框为579 bp,编码有193个氨基酸,提交GenBank(EU559709)。同时,试验采取半定量RT-PCR法,以18S rRNA为内标,研究了Mighty基因在仔猪18个组织中的分布和mRNA的表达水平。Gene Mighty is a novel promyogenie factor in skeletal myogenesis. The coding sequence of porcine Mighty gene was amplified based on the in silico sequence information in the present study, which was submitted to GenBank （EU559709）. This sequence consisted of 874 bp. The open reading frame within the sequence consisted of 579 bp, and the predicted protein sequence was 193 amino acid residues. We analyzed the tissue expression of porcine Mighty gene by the semi - quantitative RT - PCR, using 18S rRNA as the internal standard.

关 键 词：MIGHTY 猪 RT—PCR 基因克隆 组织表达 分布 

分 类 号：S828[农业科学—畜牧学]