分子标记在丛枝菌根研究中的应用  

Application of Molecular Markers in Arbuscular Mycorrhizal Studies

作　　者：孙向伟[1] 王晓娟[1] 张贵启[1] 高飞翔[2] 沈禹颖[1] 金樑[1] 

机构地区：[1]兰州大学草地农业科技学院,甘肃兰州730020 [2]内蒙古巴彦淖尔市气象局,内蒙古临河015000

出　　处：《云南农业大学学报（自然科学版）》2009年第2期278-285,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家重点基础研究发展计划项目(2007CB106804);兰州大学引进人才专项基金项目(582402,582403)

摘　　要：作为一类具有重要生态学意义的微生物类群,丛枝菌根(Arbuscular mycorrhizal,AM)真菌在自然生态系统中的功能已经成为当前生态学研究的热点问题之一,其中,AM真菌的分类鉴定是菌根学研究的重要组成部分。鉴于经典的菌根真菌鉴定技术的局限性,本文围绕以PCR为基础的分子生物学技术,探究RAPD、RFLP、SSCP、AFLP等在AM真菌分类鉴定、亲缘关系分析、多态性测定以及群落结构组成等方面的应用及其取得的成果,并对不同分子生物学技术之间的优缺点进行对比。在此基础上,对利用分子标记技术开展AM真菌研究的前景进行展望。Arbuscular mycorrhiza （AM） are ubiquitous symbionts between the fungus and host plant, which could enhances plant growth through increasing nutrient acquisition, alleviating environmental stresses and increasing biomass production. The function of AM fungi in natural ecosystems is one of the hotspots in ecology research. Thereinto, the identification of AM fungi is an important field in my corrhizal studying. According to the limitation of the traditional methods, the latest molecular markers techniques have been widely used in this field. In order to develop the research on AM fungi identification, RAPD, RFLP, SSCP, AFLP, and other molecular markers techniques were discussed. Relation- ship analysis, determination of the polymorphism, community structure and composition, the advantages and disadvantages between different techniques of molecular markers were discovered. On the basis of this, the perspective about using molecular biology techniques in AM fungi study was discussed.

关 键 词：丛枝菌根真菌 分子标记 RAPD PCR RFLP 

分 类 号：Q933[生物学—微生物学]