HPLC法测定利咽泡腾颗粒剂中绿原酸的含量  

HPLC Determination of Content of Chlorogenic Acid in Liyan Effervescent Granule

作　　者：黄薇薇[1] 徐进[1] 谭家风[1] 

机构地区：[1]南京医科大学附属儿童医院,江苏南京210008

出　　处：《南京中医药大学学报》2009年第2期148-149,共2页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：南京市科技发展计划项目(200601054)

摘　　要：目的研究利咽泡腾颗粒中绿原酸的含量测定。方法采用高效液相色谱法进行测定。ODS柱，甲醇：水：冰醋酸（30：70：0．5）为流动相，流速1mL/min，检测波长327nm。结果绿原酸在5～75μg范围内线性良好，相关系数r=0．9997，回收率为99．43％，RSD=0．53％。结论本法准确可靠，可作为其质量控制的指标。OBJECTIVE To investigate into content determination of chlorogenic acid in Liyan Effervescent Granule. METHOD HPLC method was used in the investigation： ODS column; methanol：water： glacial acetic acid （30：70：0.5, China） as the mobile phase; flow rate at l ml/min. ;detection wavelength at 327 nm. RESULT Within the range of 5 - 75μg, chlorogenic acid assumed fine linear features, with a correlation coefficient of 0.999 7, a recovery rate of 99.43 % and RSD = 0.53 %. CONCLUSION This method is accurate and reliable and can be used in the quality control of Liyan Effervescent Granule.

关 键 词：利咽泡腾颗粒 绿原酸 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]