龟纹瓢虫(Propylea japonica (Thunberg))卵子发生的组织学研究  

Oogenesis in Propylea japonica (Thunberg)

作　　者：张天澍[1,2] 李恺[1] 张丽莉[1] 王斌[1] 

机构地区：[1]华东师范大学生命科学学院 [2]华东师范大学资源与环境科学学院,上海200062

出　　处：《西北农林科技大学学报（自然科学版）》2009年第3期175-180,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：上海市青年科技启明星计划项目(04QMX1418)

摘　　要：【目的】系统观察龟纹瓢虫(Propylea japonica (Thunberg))卵子发生过程,为进一步了解龟纹瓢虫的生殖生物学奠定基础。【方法】对处于不同发育时期的龟纹瓢虫卵巢做石蜡切片,光镜下观察其卵子发生过程。【结果】第1阶段,在卵原区可见分化的卵母细胞和滋养细胞,卵母细胞正进行减数第一次分裂;第2阶段,卵母细胞向生长区移动,细胞体积逐渐增大,细胞核形成胚泡,前滤泡细胞逐渐分布于卵母细胞之间;第3阶段,卵母细胞体积明显增大,呈圆球形,前滤泡细胞分化形成滤泡细胞,呈单层扁平细胞包围卵母细胞,胚泡移动到卵母细胞的一侧;第4阶段,滤泡细胞呈立方形,此时卵母细胞仍然通过营养索与滋养细胞相连;第5阶段,卵母细胞不再与滋养细胞连接,滤泡细胞由立方形变为扁平状,在卵母细胞质的边缘处出现细小的卵黄颗粒;第6阶段,卵黄颗粒逐渐充满整个卵母细胞,胚泡退化;第7阶段,卵母细胞表面形成卵黄膜和卵壳,滤泡细胞逐渐与卵母细胞分离,卵母细胞发育为成熟的卵子。【结论】龟纹瓢虫卵子发生过程可明显划分为卵母细胞分化期、卵母细胞生长期、卵黄形成及卵子成熟期3个时期,并可进一步分为7个阶段。[Objective] The study was intended to observe the oogenesis of Propylea japonica （Thunberg） and to provide theoretical basis for reproductive biology of Propylea japonica. [Method] Ovaries in different developmental stages were studied by paraffin sectioning. [Result] （1） Oocytes and nurse cells were located in the germarium; first meiotic division occurred at this stage; （2） Oocytes grew in size and moved towards the vitellarium. The nucleus of the oocyte transformed into germinal vesicle. And the pre- follicle cells were located between the oocytes; （3） Oocytes became globosity and the monolayer follicle cells were flat surrounding the oocyte,the germinal vesicle moved into the periplasm; （4） Follicle cells became cubic in shape, and the nurse cells still connected with the oocytes by the nutritive cord at this stage; （5） The nutritive cord disappeared,and the follicle cells became flat and some yolk granules appeared on the edge of cytoplasm; （6） Ooplasm was gradually filled up with yolk granules, and the germinal vesicle breakdown occurred; （7） The vitellin member and egg shell formed and encircled the oocyte. [Conclusion] Oogenesis in the adult stage of Propylea japonica （Thunberg） could be divided into 3 phases and 7 stages.

关 键 词：龟纹瓢虫 卵子发生 卵母细胞 

分 类 号：Q965[生物学—昆虫学]