阴道乳杆菌诱导细菌性阴道病患者β-防御素2表达及其抗菌活性的研究  

Study on human β-defensin 2 mRNA expression,its antimicrobial activity and effects of Lactobacilli

作　　者：姚蔚[1] 刘佳明[2] 林刚[1] 

机构地区：[1]温州医学院生物学实验教学中心,浙江温州325035 [2]温州医学院环境与公共卫生学院,浙江温州325035

出　　处：《中国微生态学杂志》2009年第2期156-158,共3页Chinese Journal of Microecology

基　　金：浙江省医药卫生科学研究基金(2006B108)

摘　　要：目的观察阴道乳杆菌诱导阴道黏膜细胞表达β防御素-2(hBD-2)的情况,检测其表达,观察hBD-2的体外抗菌活性。方法分为3组,即健康对照组(n=10)、BV组(n=10)和阴道乳杆菌干预组(n=8)。采用RT-PCR半定量法,检测阴道组织hBD-2 mRNA的水平。用琼脂糖弥散法检测其抗菌活性。结果与健康对照组相比,BV组、阴道乳杆菌干预组hBD-2 mRNA的水平明显升高(P<0.05);阴道乳杆菌干预组hBD-2 mRNA的水平较BV组增高(P<0.05);琼脂糖弥散法显示,hBD-2对金黄色葡萄球菌、大肠埃希菌有明显的杀灭效应。结论阴道乳杆菌干预对BV患者治疗可以使hBD-2 mRNA表达水平上调,提示其在维系正常妇女生殖道内环境稳定、宿主天然抗感染机制中起着重要的作用。Objective To investigate the changes of the mRNA expression of hBD-2 in the vaginal tissues in women with bacterial vaginosis,and observe the antimicrobial activity of recombinant hBD-2. Method Women were divided into three groups, healthy group （ n = 10）, BV group （ n = 10） and Lactobacilli treatment group （ n = 8 ）. The level of expression of hBD-2 mRNA was detected by semi-quantitative RT-PCR analysis; the antimicrobial activity of recombinant hBD-2 was assessed by Lehrer diffusion test. Result Compared with healthy group,the expressions of hBD-2 mRNA significantly increased in BV group and Lactobacilli treatment group （ P 〈 0.05 ） ; the expression of hBD-2 mRNA was notably higher in treatment group than that of BV group （P 〈0.05 ）. Recombinant hBD-2 in supemate showed antimicrobial activity. Conclusion Treatment of vaginal Lactobacilli can up-regulate hBD-2 mRNA expression in vaginal tissue, which suggests that vaginal Luctobacilli may play an important role in the defense mechanisms of human genital tract.

关 键 词：人Β防御素-2 阴道组织 乳杆菌 抗菌活性 

分 类 号：R378.992[医药卫生—病原生物学]