金匮肾气丸对雄激素部分缺乏大鼠血清睾酮及StAR蛋白mRNA表达的影响  

The effect of Jinguishenqi pill on the level of serum testosterone and mRNA expression of StAR in partial androgen deficiency rats

作　　者：吴天浪[1] 张培海[1] 钟钦 曲晓伟[1] 李俊涛[3] 常德贵[1] 张蜀武[1] 王久源[1] 

机构地区：[1]成都中医药大学附属医院男科,成都610072 [2]广州邮电医院外科 [3]河南中医学院第一附属医院男科

出　　处：《中国男科学杂志》2009年第2期28-30,共3页Chinese Journal of Andrology

摘　　要：目的探讨金匮肾气丸对雄激素部分缺乏模型大鼠血清睾酮及StAR蛋白mRNA表达的影响。方法将40只SD大鼠采用腹腔注射环磷酰胺(20mg/kg·d^(-1))复制模型后,随机分为4组,即模型组、金匮肾气丸高、中、低剂量组,每组各10只,分别采用蒸馏水及金匮肾气丸水溶液高、中、低剂量灌胃,治疗28d后,观察血清睾酮及睾丸StAR蛋白mRNA表达。结果与模型组(98.33±8.36)ng/dl比较,金匮肾气丸高剂量组(301.17±46.90)ng/dl、中剂量组(215.46±26.73)ng/dl血清睾酮显著性升高,差异有统计学意义(P<0.05,P<0.01);金匮肾气丸高剂量组(11.08±1.45)、中剂量(10.47±1.26)、低剂量组(7.30±1.08)之间睾丸StAR蛋白mRNA表达水平分别与模型组(4.87±0.95)比较差异有统计学意义(P<0.01)。结论金匮肾气丸可提高雄激素部分缺乏大鼠血清睾酮水平,其机制可能是通过提高睾丸StAR蛋白mRNA表达水平实现。Objective To investigate the effect of Jinguishenqi pill on the level of serum testosterone and mRNA expression of StAR in partial androgen deficiency rats. Methods Total of 40 SD rat models established by injecting with cyclophosphamide （20mg/kg·d^-1） in cavitas were randomly divided into model group（10 rats）, treated groupl withhigh dosage of Jingui Shenqi Pill（10 rats）, treated group2 with middle dosage（10 rats） and treated group3 with low dosage（10 rats）, All rats in treated group were affused into the stomach with the Jinguishenqi Pill solution. After 28 days treatment, the level of serum testosterone and mRNA expression of StAR were detected. Results Compared as that of model group （98.33~8.36）ng/dl, there was a significant change in the level of serum testosterone of treated groupl or treated group2 （P〈0.05, P〈0.01）. A obvious change in mRNA of StAR was also found between model group and treated group（P〈0.01）. Conclusion Jinguishenqi pill might modulate the level of serum testosterone in partial androgen deficiency rats by upregulating the expression of STAR.

关 键 词：肾气丸 大鼠 睾酮 StAR蛋白 

分 类 号：R285[医药卫生—中药学] R-332[医药卫生—中医学]