二温式聚合酶链反应鉴别诊断迟缓爱德华氏菌病  

Idntification of Edwarsiella tarda Strains by Two-Temperature PCR

作　　者：邓显文[1] 谢芝勋[1] 谢丽基[1] 刘加波[1] 谢志勤[1] 庞耀珊[1] 余晓丽[2] 

机构地区：[1]广西兽医研究所,广西南宁530001 [2]广西水产研究所,广西南宁530021

出　　处：《水生态学杂志》2009年第2期158-160,共3页Journal of Hydroecology

基　　金：广西科技攻关项目(桂科攻0428001-2)

摘　　要：根据迟缓爱德华氏菌株23SrDNA基因,设计合成一对引物XZE15、XZE16,建立了二温式聚合酶链反应(PCR)鉴别诊断迟缓爱德华氏菌株的技术。特异性试验表明,对3株迟钝爱德华氏菌株进行PCR扩增出与预期大小相一致的284bp的特异性片段,但对3株钻鱼爱德华氏菌及其他对照鱼病病原体核酸模板的PCR扩增不出现任何条带;敏感性试验结果显示,该二温式PCR可以检测到10pg的迟钝爱德华氏菌DNA。A pair of primer XZE15 and XZEI6 was designed and synthesized according to the conserved gene＇ sequences of 23S rDNA of Edwarsiella tarda , and then reaction parameters were optimized to develop two-temperature polymerase chain reaction （PCR） to simultaneously identify E. tarda. The results showed that only of 284pblong DNA fragment for three E. tarda strains was amplified, and no DNA fragments for three Edwarsiella ictalur strains and other fish pathogenic viruses and bacteria were amplified by this two-temperature PCR. And 10 pg DNA of E. tarda was detected by this two-temperature PCR.

关 键 词：二温式PCR 鉴别 爱德华氏菌 迟钝爱德华氏菌 

分 类 号：S941.4[农业科学—水产养殖]