扶正解毒含药血清对硫化镍诱导人支气管上皮细胞转化的保护作用  

Protective Effect of Fuzheng Jiedu Decoction(FJD)-containing Serum on Transformation of Human Bronchus Epithelial Cell Induced by Nickel Sulfide

作　　者：王婷[1] 殷建中 潘廷丽 朱玉真[1] 孙应彪[1] 吕晓云[1] 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]金川有色金属公司总校,甘肃金昌737103 [3]金川有色金属公司职工医院,甘肃金昌737103

出　　处：《工业卫生与职业病》2009年第1期21-24,共4页Industrial Health and Occupational Diseases

基　　金：教育部博士点专项科研基金(No.20050730031)

摘　　要：目的探讨硫化镍诱导人支气管上皮(16HBE)细胞恶性转化作用以及扶正解毒汤(FJD)的保护作用,研究硫化镍致癌的分子机制以及扶正解毒汤对防癌治癌的作用。方法用不同浓度的硫化镍在体外多次处理16HBE细胞,利用刀豆凝集素A(ConA)凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定;测定16HBE细胞内镍离子和自由基含量,同时加入扶正解毒含药血清,观察其保护作用。结果硫化镍可诱导16HBE细胞的恶性转化,转化细胞的生长速度加快、排列紊乱、失去接触抑制、呈交叉重叠生长、转化率呈剂量—效应关系。转化细胞可被低浓度ConA凝集并在软琼脂内生长。硫化镍暴露组细胞内镍离子和自由基含量明显增加。中、高剂量的扶正解毒含药血清可通过细胞内、外途径发挥抗氧化、清除自由基效应。结论硫化镍有较强的诱导16HBE细胞恶性转化的能力,具有致癌性。FJD血清对于硫化镍诱导的16HBE细胞恶性转化具有保护作用。Objective To study the protective effect of FJD on malignant transformation of human bronchus epithelial cells （16HBE） induced by nickel sulfide （NiS）. To study molecular mechanism of nickel carcinogenesis and cancer prevention of FJD. Methods 16HBE cells were treated repeatedly in vitro by different concentrations of NiS, the malignancy identification of the transformation of 16HBE cells was carried out by the tests of ConA and the growth on soft agar culture. The contents of Ni^2＋and free radicals in 16HBE cells were measured. FJD-eontaining serum was added. The impact on NiS was observed. Results NiS could induce the malignant transformation of 16HBE cells. The growth velocity of transformed cells was accelerated. The transformed colonies exhibited in extensive random orientatiofi, grew in overlapping order and lost contact inhibition. The frequency of transformation showed a dose-response relation at the experimental concentrations. The transformed cells could be agglutinated by low concentration of ConA and could grow in soft agar. The contents of Ni^2＋ and free radicals in NiS exposed cells increased significantly. Medium and high dosages of FJD-containing serum could play roles in anti-oxidation and diminishing free radicals either in intracellular and in extracellular routes. Conclusions NiS could strongly induce the malignant transformation of 16HBE cells and might be carcinogenic to human. FJD-containing serum might have protective effect on malignant transformation of 16HBE cells induced by NiS.

关 键 词：硫化镍 人支气管上皮细胞 扶正解毒含药血清 

分 类 号：R13[医药卫生—劳动卫生]