假奓包叶的抗菌活性成分  

Antibacterial constituents of extracts of the aerial parts of Discocleidion rufescens

作　　者：田冶[1,2] 汤海峰[1] 邱峰[2] 王晓娟 薛改进[2] 李军[2] 

机构地区：[1]第四军医大学西京医院,陕西西安710032 [2]沈阳药科大学中药学院,辽宁沈阳110016 [3]第四军医大学口腔医院,陕西西安710032

出　　处：《沈阳药科大学学报》2009年第3期191-195,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的研究假奓包叶的抗菌活性成分。方法在抗菌药理活性跟踪下,应用多种色谱技术进行化合物分离,运用波谱分析法和化学手段鉴定其结构。结果从具有明显抗菌活性的部位分离得到了8个化合物,分别鉴定为洋芹素(1)、木樨草素(2)、穗花杉双黄酮(3)、丹皮酚(4)、脱镁叶绿素a(5)、132S-羟基脱镁叶绿素a(6)、β-谷甾醇(7)、豆甾醇(8)。结论化合物4-6为首次从假奓包叶属植物中得到。药理实验表明,化合物1-6有一定的抑制大肠杆菌的活性;化合物4-6为首次从假奓包叶属植物中得到。药理实验表明,化合物1-6有一定的抑制大肠杆菌的活性;其中化合物3-6还具有抑制金黄色葡萄球菌的活性,化合物4活性最显著。Objective To screen effective constituents of the aerial parts of Discocleidion rufescens against bacteria. Methods Compounds were isolated by bioassay-guided fractionation method with a combination of extraction and partition as well as multi-chromatography. Their structures were determined by spectral analy- sis and chemical evidences. Results Eight compounds were isolated from the antibacterial active fraction and identified as apigenin （ 1 ）, luteolin （ 2 ）, amentoflavone （ 3 ）, paeonol （ 4 ）, pheophytin a （ 5 ）, 13^2S- hydroxypheophytin a （6） ,β-stitosterol （ 7 ） and stigmasterol （ 8 ）. Conclusions Compounds 4 - 6 are isolated from the plant of genus Discocleidion for the first time. The pharmacological results shows that compounds 1 -6 exhibit certain inhibitory function. The activities of compound 4 are the highest.

关 键 词：假穸包叶 抑菌作用 化学成分 

分 类 号：R284.1[医药卫生—中药学]