ROSA26小鼠中枢神经系统半乳糖苷酶的表达与分布  

The expression and distribution of β-galactosidase in the central nervous system of ROSA26 mouse

作　　者：刘秀玲[1] 苏阳[1] 方杰[1] 肖明[1] 韩群颖[1] 丁炯[1] 

机构地区：[1]南京医科大学人体解剖学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2009年第3期308-311,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助(BK2006576和BK2006232)

摘　　要：目的:观察成年ROSA26小鼠中枢神经系统半乳糖苷酶(X-Gal)的表达与分布。方法:应用X-Gal组织化学结合尼氏染色及免疫组织化学双标技术研究β-gal在该嵌合体脑内的表达与分布特征。结果:X-Gal组织化学染色显示在该线系小鼠脑和脊髓中均广泛表达转基因β-gal活性产物,但以灰质为主。X-Gal和尼氏双重染色表明,β-gal在神经元、室管膜细胞和脉络丛上皮细胞中均有表达。免疫组化进一步显示β-gal广泛表达于神经元中,但在星形胶质细胞中则未见表达。结论:ROSA26小鼠中枢神经细胞稳定而广泛的表达β-gal,此转基因模式动物将可用于神经细胞培养、移植及其它相关神经生物学研究。Objective：To observe the expression and distribution of β-galactosidase in the central nervous system （CNS） of aduh ROSA26 mouse. Methods：X-Gal histochemistry combined with Nissl＇s or immunohistochemistry double-staining was performed to localize β-gal activity in the ROSA26 mouse CNS. Results： X-Gal histochemistry staining showed that transgenic β-gal reaction products were throughout the central nervous system, but mainly located at the grey matter. X-Gal and Nissl＇s double-staining showed that β-gal expressed in neurons, ependymal cells and choroid plexus cells. X-Gal histoehemistry combined with immunohistochemistry further revealed that β-galactosidase was ubiquitous expression in neurons except astrocytes. Conclusion：β-gal is extensively and stably expressed in the ROSA26 mouse CNS. This transgenic line may be useful in nerve cell culture, transplantation and other neurobiological studies.

关 键 词：β-gal X-Gal 转基因小鼠 中枢神经系统 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]