缓冲体系和金属离子对漆酶催化活性的影响  

Effect of Buffer Solutions and Metal Ions on the Laccase Activity

作　　者：方华[1] 黄俊[1] 陈曌[1] 丁莉芸[1] 

机构地区：[1]武汉理工大学光纤传感技术研究中心,武汉430070

出　　处：《武汉理工大学学报》2009年第5期27-30,共4页Journal of Wuhan University of Technology

基　　金：国家自然科学基金(60537050,60377032)

摘　　要：探讨了3种不同的缓冲体系和6种常见的金属离子对漆酶催化活性的影响。结果表明:不同的缓冲体系对漆酶催化活性的影响较大。在pH为2.0的磷酸氢二钾-柠檬酸缓冲体系(0.1 mol/L)中漆酶的催化活性最大。金属离子对漆酶催化活性的影响与金属离子的种类和浓度有关,在浓度低于0.1 mmol/L时,Pb2+、Ag+、Hg2+对漆酶的催化活性起激活作用,高于0.1 mmol/L时起抑制作用。在浓度低于20 mmol/L时,Mg2+、Zn2+、Cu2+对漆酶的催化活性起激活作用,高于20 mmol/L时起抑制作用。The effects on ABTS, oxidation catalyzed by laccase with different buffer solutions and metal ions （ Mg^2 ＋ , Zn^2＋ ,Cu^2＋ , Pb^2＋ , Ag^＋ , Hg^2 ＋ ） were investigated by Spectrophotometer. The results show that the best suitable buffer is K2HPO4- C6H8O7 buffer solution and the optimal pH was 2.0. The effects of metal ions on laccase activity are related to the type and concentration of metal ions. Pb^2＋, Ag^ ＋ , Hg^2 ＋ activated the activity of laccase when the concentrations are lower than 1 mm, however, once the concentrations were higher than 1 retool/L, the activity was inhibited. As for Mg^2＋ , Zn^2＋ , Cu^2 ＋ , the inhibition was cocurrcd only when the concentrations were higher than 20 mmol/L.

关 键 词：漆酶 缓 中体系 金属离子 激活作用 抑制作用 

分 类 号：Q554[生物学—生物化学]