比浊法测定红霉素生物效价及其高通量测定方法探讨  

The turbidimetric method for erythromycin bioactivity and the discussion of the high throughput measurement

作　　者：谭俊[1] 高勇生[1] 郝玉有[2] 庄英萍[2] 储炬[2] 

机构地区：[1]江西农业大学,南昌330045 [2]华东理工大学生化反应器工程国家重点实验室国家生化工程技术研究中心,上海200237

出　　处：《中国抗生素杂志》2009年第3期170-172,共3页Chinese Journal of Antibiotics

基　　金：十一五863重点项目(2006AA020302和2006AA020304)

摘　　要：基于比浊法测定抗生素体外抑菌活性原理,以红霉素发酵液为研究对象,采用96孔微孔板培养检定菌,直接用酶标仪检测各孔的光密度,定量分析发酵液中红霉素含量,并与管碟法比较。本文建立的抗生素生物效价检测方法具有快速、高效、样品和试剂用量少、结果可靠等优势,适合在有检测大量样品和快速获取结果的工作中推广。Based on the traditional turbidimetric method, 96-well mierotiter plates were adopted to cultivate the indication bacterium. The culture broths were determined by an ELISA Reader. The titers of erythromycin were calculated based on the relationship with the absorbence. The results were compared with that obtained by inhibition zone and traditional turbidimetric methods. The established method has the characteristics of speediness, high efficiency, accurate, cost-effective and could be applied in batch and fast identification analysis.

关 键 词：红霉素 高通量 生物效价 比浊法 

分 类 号：TQ465.5[化学工程—制药化工]