海藻酸钠修饰的水溶性壳聚糖药物载体  

Drug Carrier Water-soluble Chitosan Modified by Alginate

作　　者：王春[1] 孙胜玲[1] 肖惠宁[1] 何北海[1] 杨连生[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《安徽农业科学》2009年第6期2341-2342,2354,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(20776054)

摘　　要：[目的]改善水溶性壳聚糖(WSC)作为蛋白药物载体的作用。[方法]采用离子交联法制备海藻酸钠修饰的WSC纳米粒子并测定粒子的TEM、粒径和zeta-电位。[结果]WSC、负载BSA的WSC以及海藻酸钠修饰的WSC纳米粒子均呈球状,纳米粒子的粒径基本在200 nm以内,WSC和海藻酸钠修饰的WSC纳米粒子的粒径较小,在100 nm左右;而负载BSA的WSC粒子的粒径较大,在200 nm左右。当海藻酸钠浓度从0.1 mg/m l增大到0.3 mg/m l时,WSC纳米粒子的BSA负载率从32%增加到94%,载药量从4.8%增加到24.0%。在3 d内,WSC和海藻酸钠修饰的WSC纳米粒子的BSA释放量分别是40%和13%。[结论]海藻酸钠与WSC之间产生了较强的化学交联作用,且海藻酸钠的修饰提高了WSC纳米粒子的药物负载能力。[Objective] The purpose of the research was to improve the function of water-soluble chitosan （WSC） as drug carrier. [Method] The WSC nanoparticles modified by alginate were prepared by tripolyphosphate and their TEM, particle sizes and zeta-electric potential were determined. [ Result] WSC, WSC loading BSA and WSC nanoparticles modified by alginate were spherical. The particle sizes of nanoparticles were basically smaller than 200 nm, that of WSC and WSC nanoparticles modified by alginate were smaller, being about 100 nm and that of WSC loading BSA were bigger, being about 200 nm. When the concn, of alginate was increased from 0. 1 mg/ml to 0.3 mg/ml, the rate of WSC nanoparticle loading BSA was increased from 32% to 94% and its drug loading was increased from 4.8% to 24.0%. In 3 d, the amounts of BSA released from WSC and WSC nanoparticle modified by alginate were 40% and 13% resp. [ Conclusion] There occurred relatively strong chemical crosslinking effect between alginate and WSC and the modification of alginate enhanced the drug leading ability of WSC nanoparticle.

关 键 词：水溶性壳聚糖 海藻酸钠 纳米粒子 牛血清蛋白 蛋白质释放 

分 类 号：O548[理学—物理]