正交设计优选黄芪多糖的微波辅助与传统提取工艺研究  

Study on Microwave-assisted Extraction and Conventional Extraction Astragalus-polysaccharose by Orthogonal Design

作　　者：张萍[1] 梁新丽[1] 廖正根[1] 赵国巍[1] 赖珺[1] 戴春兰[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《江西中医学院学报》2009年第1期33-35,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家十一五科技支撑计划资助项目(2006BAIO9B07-05);江西省卫生厅中医药科研基金资助项目(2007A018);江西省教育厅科学技术研究项目(GJJ08327)

摘　　要：目的:以水为提取溶剂,比较微波辅助与传统提取对黄芪多糖的影响研究。方法:采用正交实验设计,蒽酮-硫酸比色法测定其含量,优选微波辅助与传统提取黄芪的工艺,比较两种提取工艺对黄芪多糖的影响。结果:优化传统提取条件为料液比12∶1,提取时间60min,提取2次,黄芪多糖提取率为89.34%;微波辅助提取条件为料液比12∶1,提取时间15min,微波功率800W,黄芪多糖提取率为93.02%。结论:微波辅助提取时间短、有效成分提取率高,可用于黄芪多糖的提取工艺。Objective：Comparative study on extracting astragalus-polysaccharose from conventional extraction and microwave-assisted by water. Methods：Orthogonal test was used, Polysaccharide content was determined with anthrone-sulphuric acid colorimetry. Results：The optimum conventional extraction procedure was as fallow： 12 times of water decocting 60 min ,2 times, the polysaccharide extraction rate was 89.34% ;The optimum microwave-assisted extraction procedure was：12 times of water abstraction 15 min, microwave irradiation （800W） 2 times,the extraction rate was 93.02%. Conclusion：The microwave technique could be a better method for polysaccharides extraction in the Radix astragali.

关 键 词：正交设计 黄芪 多糖 微波提取 传统提取 

分 类 号：R284.2[医药卫生—中药学]