复合纤维素降解菌对城市落叶的降解研究  

Study of Disposing City Defoliation by Cellulose Degradation Bacteria System

作　　者：冯蕾[1] 胡滨[1] 赵艳丽[1] 吴兆亮[1] 

机构地区：[1]河北工业大学化工学院,天津300130

出　　处：《安徽农业科学》2009年第6期2399-2401,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省科技厅河北省科学技术研究与发展指导计划项目(072156120)

摘　　要：[目的]研究了纤维素降解菌处理落叶的方法,以解决目前城市落叶难于处理的问题。[方法]以落叶为发酵过程中唯一的碳源,研究不同起始pH值对降解过程中的pH值和酶活的影响,确定发酵最佳起始pH值;比较不同培养方式对降解效果的影响;测定发酵过程中产酶、产还原糖的变化;通过干物质的变化及其分解率来确定降解效果。[结果]试验得出,发酵的最佳起始pH值为6.0。静瓶培养比摇瓶培养降解效果更明显。在发酵第5天出现最高CMC酶活,达到9.012 U;第7天滤纸酶活最高,达到14.806 U。发酵结束后,干物质的分解率最高可达到43.42%。复合菌系对发酵液的pH值有良好的调节作用。[结论]纤维素降解落叶的方法不仅能够产还原糖还能够有效减少干物质量,对实际应用有积极的意义。To study the method of disposing the defoliation by cellulose decomposing microorganism is to solve the problems of dealing with the defoliation. The optimum initial pH value was 6.0 when city defoliation was the only carbon fountain. Cuitivated under static condition was better than shaking condition. The highest CMCase activity was 9. 012 U in the 5th day and the highest PFAase activity was 14. 806 U in the 7th day. The degradation ratio of dry mass reached 43.42%. The cellulose degradation bacteria system can change the pH vaule to neutrality after fermentation. This method which can produce reducing sugar as well as allay dry mass is very helpful and has positive Significance.

关 键 词：纤维素降解 城市落叶 酶活 

分 类 号：S182[农业科学—农业基础科学]