咖啡酸锗对小鼠肝癌H22端粒酶活性及凋亡的影响  

Effects of Caffeic Acid Ge on H22 Cancer Cells Telomerase Activity and Apoptosis in Mice

作　　者：张晓春[1] 肖纯[1] 乔玉丹[1] 刘春花[1] 周志愉[1] 林波[1] 

机构地区：[1]江西中医学院基础医学院,南昌330006

出　　处：《江西中医学院学报》2009年第1期48-49,共2页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30460148);江西省教育厅科技资助项目[No.赣教技字(2oo7)252号]

摘　　要：目的:探讨咖啡酸锗对小鼠肝癌H22端粒酶活性及细胞凋亡的影响。方法:建立小鼠肝癌H22移植瘤模型,应用咖啡酸锗腹腔注射给药后,通过电镜观察H22细胞凋亡的情况以及用ELISA法半定量检测端粒酶活性。结果:咖啡酸锗能诱导H22细胞凋亡以及抑制端粒酶的活性,并与药物浓度有关。结论:咖啡酸锗能显著抑制H22细胞端粒酶的活性,抑制其细胞增殖,诱导其细胞凋亡。Objective：To investigate the effects of Caffeic acid Ge on telomerase activity and apoptosis on H22 cancer cells in mice. Methods ： Establishing model of H22 cancer cells in mice and after the treatment for abdominal using Caffeie acid Ge. Cell apoptosis was observed by transmission electron microscopy, and mice telomerase enzyme linked immunoassay（小鼠端粒酶 TE ELISA）was used to assay telomerase activity of H22 cells. Resuhs：Caffeic acid Ge could induced H22 cells apoptosis and inhibit telomerase activity by different concentrations. Conclusion：Caffeic acid Ge could inhibit H22 cells telomerase activity and cell proliferation obviously and could also induce apoptosis.

关 键 词：咖啡酸锗 H22细胞 端粒酶 细胞凋亡 

分 类 号：R965[医药卫生—药理学]