马MxA基因第13外显子的多态性研究  

Polymorphism Analysis of the Thirteenth Exon of Equine MxA Gene

作　　者：萨仁高娃[1] 菊林花[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《安徽农业科学》2009年第6期2411-2412,共2页Journal of Anhui Agricultural Sciences

基　　金：内蒙古自治区自然科学基金资助项目(200508010413)

摘　　要：[目的]研究4个品种马MxA基因第13外显子的多态性。[方法]根据GenBank中已公布的MxA基因序列设计引物,利用PCR技术从三河马、锡尼河马、乌审马和巴尔虎马血液基因组DNA中扩增MxA第13外显子基因片段,利用PCR-SSCP方法检测该基因片段多态性并进行测序。[结果]只有乌审马MxA基因第13外显子存在多态性,第2 018位点发生了突变,鸟嘌呤(G)→腺嘌呤(A),由此导致氨基酸发生改变,由谷氨酸(G lu)→丙氨酸(A la)。[结论]该研究为探讨马MxA蛋白基因抗病毒作用奠定了基础。[ Objective ] To explore the polymorphism of the thirteenth exon of MxA gene in 4 species of horse. [ Method] The thirteenth exon gene fragments of MxA were amplified from genomic DNA of Sanhe horse, Xinihe horse, Wushen horse and Baerhu horse by PCR with the primers designed according to the MxA sequence published in GenBank; the polymorphism of this gene was then detected by PCR-SSCP and the products were sequenced. [ Result] The polymorphism of the thirteenth exon of MxA gene appeared only in Wushen horse, the 2 018 nt of which mutated from guanine （G） to adenine （A） and the corresponding amino acid of which changed from glutamate （Glu） to alanlne （Ala）. [ Conclusion ] This study provided a basis for exploring the antiviral effect of MxA protein.

关 键 词：马 MxA基因 多态性 PCR-SSCP 

分 类 号：S821.2[农业科学—畜牧学]