蚯蚓血细胞微核试验对汞·镉遗传毒性的研究  

Study on the Genetic Toxicities of Hg and Cd through the Micronucleus Test of Earthworm Blood Cell

作　　者：王盛权 周汝敏[1] 张云峰[1] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650092

出　　处：《安徽农业科学》2009年第6期2449-2451,2695,共4页Journal of Anhui Agricultural Sciences

基　　金：国家科技部十五科技支撑项目(2007BAD87B12)

摘　　要：[目的]探讨Hg和Cd的遗传毒性。[方法]以赤子爱胜蚓为试验材料,采用滤膜法探讨Hg和Cd单一污染对蚯蚓血细胞微核的影响。[结果]在一定浓度范围内,Hg和Cd单一污染对蚯蚓血细胞微核的产生均有显著影响。微核率随处理浓度的增加呈现先上升后下降的趋势。回归分析显示,Hg在0-25.5 mg/L的浓度范围内,微核率与处理浓度呈极显著正相关（r=0.93,F=224.118,P〈0.01）,在25.5-63.75 mg/L浓度范围内,微核率与处理浓度呈极显著负相关（r=0.85,F=127.255,P〈0.01）。Cd在0-115 mg/L浓度范围内,微核率与处理浓度呈极显著正相关（r=0.69,F=63.667,P〈0.01）,在115-143.75 mg/L浓度范围内,微核率与处理浓度呈极显著负相关（r=0.94,F=137.413,P〈0.01）。[结论]该研究提供了一种新的检测土壤或水中重金属污染的生物学方法。[ Objective] The aim was to discuss the genetic toxicities of Hg and Cd.[ Method ] With Eisenia foetida as tested material, the effects of single pollutions of Hg and Cd on the micronucleus ofE. foetida blood cell were discussed by filter method. [ Result] The single pol- lution of Hg and Cd all had significant effect on the production of micronucleus of E. foetida blood cell in a certain concn, range. The micronucleus rate was first increased and then decreased along with the increasing of the treated concn. The regression analysis showed that when Hg was 0 - 25.5 mg/L, the micronucleus rate showed extremely significantly positive correlation with the concn. （ r = 0.93, F = 224. 118, P 〈 0.01 ） and when Hg was 25.5 -63.75 mg/L, the micronucleus rate showed extremely significantly negative correlation with the concn. （r = 0. 85, F = 127. 255, P 〈 0.01 ）. When Cd was 0 - 115 mg/L, the micronucleus rate showed extremely significantly positive correlation with the concn. （r =0.69, F = 63. 667, P 〈 0.01 ） and when Cd was 115 -143.75 mg/L, the micronucleus rate showed extremely significantly negative correlation with the concn.（ r = 0. 94, F = 137.413, P 〈 0.01 ）. [ Conclusion ] The study provided a new biological method for detecting the pollution of heavy metal in soil or water.

关 键 词：HG Cd 蚯蚓 微核 

分 类 号：Q958.1[生物学—动物学]