大孔树脂对槐花总黄酮的吸附分离性能研究  

Research on the Adsorption and Separation Performance of Macroporous Resin on Total Flavonoid from Sophora japonica

作　　者：李云[1] 钟辉[1] 兰昌云[1] 

机构地区：[1]成都理工大学材料与化学化工学院,四川成都610059

出　　处：《安徽农业科学》2009年第6期2549-2551,2600,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]筛选对槐花总黄酮具有较好吸附和解吸能力的大孔吸附树脂并确定其最佳吸附及脱附条件。[方法]通过考察流速、温度和pH值等影响树脂吸附和脱附性能的因素,确定最佳的吸附和脱附条件。[结果]D4020型非极性大孔吸附树脂对槐花总黄酮有较好的吸附和解吸效果。最佳吸附条件为：pH值4.5,温度为15℃,上样液浓度在0.55-0.85 mg/m l,流速为2.0 BV/h。脱附条件为：70%乙醇,pH值8.5,流速为3.0 BV/h。在此条件下洗脱D4020吸附树脂,3.0 BV脱附液就可把树脂吸附的90%以上的总黄酮解吸下来,脱附的总黄酮浓度较高,且可蒸馏回收洗脱剂,降低成本。[结论]该研究为槐花总黄酮的吸附及脱附提供了科学依据。[ Objective] The aim was to screen the maeroporous adsorption resin with better adsorption and desorption ability on total flavonoid from Sophora japonica and ascertian its optimum adsorption and desorption condition. [ Method ] The influencing factors of adsorption and desorption performance of resin, such as flow velocity, temperature and pH were reviewed to ascertian its optimum adsorption and desorption condition. [ Result] D4020 type nonpolarity maeroporous adsorption resin had better adsorption and desorption effect on the total flavonoid from S. japonica. The optimum adsorption condition was pH of 4.5, temperature of 15 ℃, sample conen, of 0.55 -0.85 mg/ml and flow velocity of 2.0 BV/h. The desorption condition was 70% ethanol, pH value of 8.5 and flow velocity of 3.0 BV/h. Desorbing resin adsorption D4020 under this condition, 3.0 BV desorption solution could desorb more than 90% total flavonoid adsorbed on the resin, then the concn, of desorbed total flavonoid was higher and the eluent could be reclaimed through distillation, which could reduce the cost. [ Conclusion] The research provided scientific basis for the adsorption and desorption of total flavonoid from S. japonica.

关 键 词：吸附树脂 槐花 总黄酮 脱附 

分 类 号：S567.19[农业科学—中草药栽培]