豚鼠膀胱组织内Cajal样间质细胞及NOS表达阳性神经元的分布  

Distribution of ICC-like cells and NOS positive neurons in the bladder tissue of guinea pigs

作　　者：纪世琪[1] 王勤章[1] 丁国富[1] 

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832008

出　　处：《现代泌尿外科杂志》2009年第2期103-105,共3页Journal of Modern Urology

基　　金：国家自然科学基金(No.30860281)

摘　　要：目的探讨Cajal样间质细胞和一氧化氮合酶2(NOS2)表达阳性神经元在成年豚鼠膀胱组织内的分布。方法成年豚鼠膀胱冰冻切片,c-Kit免疫荧光和n-NOS2免疫荧光染色。结果膀胱肌层内可见c-Kit表达阳性细胞的存在,n-NOS2免疫荧光染色显示其阳性神经元广泛分布于膀胱组织内。c-Kit免疫荧光和n-NOS2免疫荧光双重染色虽未发现两者的共存,但可见c-Kit表达阳性细胞存在于NOS表达阳性神经元的附近。结论豚鼠膀胱的Cajal样间质细胞可能参与膀胱自主节律性运动的调控,提示二者可能共同参与膀胱自主节律性运动的调控,为"神经-Cajal样间质细胞-肌肉"作用单元的形成提供结构基础。Objective To study the distribution of ICC-like cells and NOS positive neuron in the bladder tissue of adult guinea-pigs. Methods Cryo-sections of bladder samples from adult guinea pigs were examined for detection of c-Kit and n-NOS2 by immunocytochemistry. Results In cross sections, c-Kit immunopositive cells were seen in the smooth muscle layers of bladder. The cells were spindle shaped with two slender processes. A large number of NOS positive nerve cells widely distributed in the bladder. Although no NOS reactive neurons were observed in the c-Kit positive cells, their cytoplasmic processes close to the NOS positive neurons were often encountered. Oonclusion ICC-like cells widely existing in the bladder of guinea pigs may be involved in the regulation of the auto-rhythm movement of bladder and perhaps are the targets of NOS positive neurons mediating the motility of guinea pig＇s bladder. The ICCs may take part in the construction of ＂neurons-ICC- like ceils-muscle＂ in bladder.

关 键 词：CAJAL样间质细胞 一氧化氮合酶 c—Kit 膀胱 

分 类 号：R392.31[医药卫生—免疫学]