木蹄层孔菌多糖对小鼠免疫功能的影响  

Immunomodulatory effects of Fomes fomentarius polysaccharides:an experimental study in mice

作　　者：高慧灵[1] 雷林生[1] 余传林[1] 朱正光[1] 陈娜娜[1] 吴曙光[1] 

机构地区：[1]南方医科大学药学院药物研究所,广东广州510515

出　　处：《南方医科大学学报》2009年第3期458-461,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(7005189)

摘　　要：目的初步探讨木蹄层孔菌多糖(FFP)的免疫调节作用。方法MTT法检测免疫细胞的代谢活力,ELISA法检测肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)、白细胞介素2(IL-2)的含量,鸡红细胞吞噬法检测巨噬细胞的吞噬功能,绵羊红细胞免疫法检测小鼠体液免疫功能。结果发现FFP(100,50,25μg/ml)可以明显促进小鼠脾细胞代谢活力,促进脾细胞分泌细胞因子TNF-α、IFN-γ、IL-2;显著增强腹腔渗出细胞代谢活力,促进腹腔渗出细胞分泌TNF-α。FFP(100、50、25mg/kg)显著增强绵羊红细胞(SRBC)所致小鼠特异性抗体生成。FFP(100、50mg/kg)可以增强小鼠腹腔巨噬细胞吞噬鸡红细胞(CRBC)的吞噬率和吞噬指数。结论木蹄多糖可促进小鼠免疫细胞分泌细胞因子TNF-α、IFN-γ、IL-2和增强小鼠的体液免疫功能及巨噬细胞的吞噬功能。Objective To investigate the immunomodulatory effects of Fomes fornentarius polysaccharides （FFP） in mice. Methods MTT assay was employed to evaluate the in vitro metabolic activity of the mouse splenocytes treated with FFP at different concentrations, and the secretion of tumor necrosis factor-α （TNF-α）, interferon-γ（INF-γ） and interleukin 2 （IL-2） from the cells were measured by enzyme-linked immunosorbent assay. The changes in the phagocytotic activity of mouse macrophage in response to FFP treatment were evaluated by phagocytosis percentage of chicken red blood cells （CRBCs）. The effect of FFP on the humoral immunity was assessed in mice immunized with sheep red blood cells （SRBCs） by measuring the serum levels of specific antibody （hemolysin） against SRBCs. Results FFP at the concentrations of 25, 50, and 100 μg/ml all significantly enhanced the metabolic activity of mouse splenocytes in vitro and increased the production of TNF-α, IFN-γ/and IL-2. FFP treatment also markedly enhanced the metabolic activity of mouse peritoneal exudate cells and TNF-α production by the cells. At the doses of 25, 50, and 100 mg/kg, FFP significantly increased serum hemolysin level in mice immunized with SRBCs, and FFP at 50 and 100 mg/kg obviously increased the capacity of mouse peritoneal macrophages in vivo for CRBC phagocytosis. Conclusion FFP can promote the secretion of TNF-α, IFN-γ and IL-2 by mouse immtmocytes and enhance mouse humoral immune response and the phagocytotic activity of the macrophages.

关 键 词：木蹄层孔菌 多糖 细胞因子 溶血素 免疫调节 

分 类 号：R285.5[医药卫生—中药学]