大鼠脑组织损伤后局部细胞免疫与神经元凋亡的关系  

Relationship between cellular immune response and apotosis of the brain neurons after brain trauma in rats

作　　者：陈克家[1] 李卫[2] 汪志刚[1] 

机构地区：[1]暨南大学附属第一医院急诊科,广东广州510630 [2]暨南大学附属第一医院神经外科,广东广州510630

出　　处：《南方医科大学学报》2009年第3期497-499,共3页Journal of Southern Medical University

基　　金：广东省医学科研基金(A2008353);暨南大学第一临床医学院科研培育专项基金

摘　　要：目的研究大鼠脑组织损伤后不同时期损伤灶局部CD4+、CD8+T淋巴细胞浸润情况的变化及其与损伤灶局部神经元凋亡之间的关系。方法制作大鼠脑组织损伤模型,应用免疫组化方法,在不同时程检测脑组织损伤灶局部CD4+、CD8+T细胞数及神经元凋亡数。结果伤后24h脑组织损伤灶局部CD4+、CD8+T细胞即有明显增加,伤后10d达到高峰,与对照组比较分别上升了15倍和20倍,伤后30d逐渐下降。同时期损伤灶局部神经元凋亡数亦呈现出类似的变化规律。相关分析显示损伤灶局部CD4+、CD8+T细胞数变化与神经元凋亡数之间呈密切的正相关关系。结论颅脑损伤后,脑组织损伤灶局部CD4+、CD8+T细胞增加非常显著,细胞免疫应答显著增强,介导损伤灶局部神经元凋亡,加重脑组织继发性损伤。Objective To investigate the relation between CD4^＋ and CD8^＋ T lymphocyte infiltration and apoptosis of the neurons in the local traumatic brain tissue after brain trauma in rats. Methods In rat models of brain trauma, the changes in the number of CD4^＋ and CD8^＋ T lymphocytes and the apoptosis of neurons in the local traumatic brain tissue were observed by immunohistoehemistry at different time points after brain trauma. Results Twenty-four hours after brain trauma, a significant increase in the number of CD4^＋ and CD8^＋ T lymphocytes occurred in the injured brain tissue, both reaching the highest levels on day 10, at the point of which the number ofCD4^＋ cells increased by about 15 folds and that of CD8^＋ cells by about 20 folds compared with the control groups. The CD4^＋ and CD8^＋ T lymphocytes both began to decrease 30 days after the injury. A similar pattern of alterations was found in the apoptosis of neurons in the local brain tissue. Correlation analysis demonstrated a close positive correlation between the changes in CD4^＋ and CD8^＋ lymphocyte numbers and the number apoptotic neurons in the injured brain tissue. Conclusions Brain trauma induces obvious increases in CD4^＋ and CD8^＋ T lymphocytes and enhanced cellular immune response in the injured brain tissue to mediate neuronal apoptosis and further exacerbate the brain tissue injuries.

关 键 词：脑组织损伤 细胞免疫应答 神经元 凋亡 

分 类 号：R392[医药卫生—免疫学]