腊样芽胞杆菌DNF409中硝酸盐还原酶基因敲除的研究  

Knockout of narGHJI,the Gene Encoding the Nitrate Reductase in Bacillus cereus DNF409

作　　者：刘卿[1] 梁运祥[1] 葛向阳[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2009年第1期43-47,共5页Journal of Huazhong Agricultural University

基　　金：国家高科技研究发展计划"863"水专项课题(003AA6010-01-01)资助

摘　　要：在腊样芽胞杆菌DNF409中,narGHJI基因负责编码呼吸过程中硝酸盐还原酶基因nar的α、β、γ、δ四个亚基。通过基因同源重组的方法,利用一段带有终止序列和Ω环的链霉素抗性片段,取代nar中的部分基因片段,实现硝酸盐还原酶的基因敲除,从而得到专一降解亚硝酸盐的突变体菌株,其硝酸盐还原酶的活性降低了80%。该菌与DNF409共同使用,可解除DNF409单独使用时亚硝酸盐严重积累的现象。The biological de-nitrification of the waste-water with high nitrate and nitrite via nitrite pathway is favorable technically and economically. The Bacillus cereus DNF409 gene narGHJ I, encoding the catalytic α,β,γ, δsubunits of the respiratory nitrate reductase, was knockouted by the insertion of a streptomycin resistance cassette containing terminal codes and gl ring frame. The nitrate reductase in narmutants activity was reduced under all tester conditions. The reductase activity in the double crossover strains was reduced about 80 %. The double crossover strains combined with DNF409 can improve the nitrite accumulation.

关 键 词：DNF409 硝酸盐还原酶 基因敲除 同源重组 

分 类 号：Q933[生物学—微生物学]