中国主要养殖罗非鱼亲缘关系的COI序列分析  

Phylogenetic Relationship of Oreochromis Tilapias Cultured in China's Mainland Based on COI Sequences

作　　者：郭奕惠[1,2] 喻达辉[1,2] 黄桂菊[1,2] 李莉好[1] 符云 叶卫 

机构地区：[1]中国水产科学研究院南海水产研究所,广州510300 [2]中国水产科学研究院水产种质资源与养殖技术重点开放实验室,广州510300 [3]国家级广东罗非鱼良种场,广州511453

出　　处：《华中农业大学学报》2009年第1期75-79,共5页Journal of Huazhong Agricultural University

基　　金：广东省科技计划重点项目(2004A20105002);广州番禺区科技计划项目(2004-Z-61-1);中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金(2006A003)资助

摘　　要：对中国主要养殖的尼罗、奥利亚、莫桑比克、杂交种尼奥（尼罗×奥利亚）、奥尼（奥利亚×尼罗）和红罗非鱼（莫桑比克×尼罗）的线粒体COI基因的部分序列进行了PCR扩增、克隆和测序分析，探讨罗非鱼种间亲缘关系和种类鉴别标记。6种罗非鱼中大部分个体均获得长586-708bp的基因序列。其碱基组成GC含量为47．7％。序列分析表明，莫桑比克罗非鱼2个个体分为2个单倍型，单倍型之间的碱基差异为1个碱基，其余几种罗非鱼均只有1个单倍型。其中，尼罗和尼奥、奥利亚和奥尼的单倍型序列相同，但种间核苷酸序列差异达4．3％-7．7％。根据聚类分析结果可将这6个（杂）种分为3个组：尼罗-尼奥，红罗-莫桑比克和奥利亚-奥尼罗非鱼组。其中前2组亲缘关系较近，与后1组亲缘关系较远。表明C01可作为罗非鱼种类判别和亲缘关系分析的重要标记。Mitochondrial COI gene sequence variation of Nile （Oreochromis niloticus）,Blue （O. aureus）, Mozambique （O. mossambicus）, Ni-ao （O. niloticus ♀ X O. aureus ♂）, Ao-ni （O. aureus ♀ XO. niloticus♂） and red tilapia （O. mossambicusXO, niloticus） was investigated in order to understand the genetic relationship among them as well as to provide interspecific molecular markers for breeding practice. As a result,the partial sequence of COI was 586-708 bp long. The base compositions with GC content was 47. 4%. Sequence analysis indicated that Mozambique tilapias had two haplotypes and the other five species each had one haplotype. Yet there was only one base substitution between haplotypes in Mozambique and no mutation was found between individuals within the other species. However, sequence divergence between species was high up to 4. 3%-7.7%. Phylogenetic analysis indicated that the six species can be divided into three groups, among which the group of Nile and Ni-ao tilapias was closely related with the group of Mozambique and the red tilapias,whereas group of blue and Ao-ni tilapias was genetically distinct from the former two groups. These observations showed that COl sequence marker can be applied to species discrimination and phylogenetic study on tilapias.

关 键 词：罗非鱼 COI基因 亲缘关系 

分 类 号：Q178.53[生物学—水生生物学] Q953[生物学—普通生物学]