大丽菊杂合花色突变体的发现及RAPD鉴定  

The Molecular Identification of a Novel Versicolor Mutant in Dahlia pinnata Cav Using RAPD Technique

作　　者：阚雪芹[1] 方向明[1] 贾俊丽[1] 郑必平[1] 谈建中[1] 

机构地区：[1]苏州大学城市建设学院,江苏苏州215123

出　　处：《北方园艺》2009年第3期75-77,共3页Northern Horticulture

摘　　要：以新发现的大丽菊花色突变体为材料,选取10个具有10个碱基长度的随机引物,对5种突变个体的基因组DNA进行了RAPD分析。结果表明：10个随机引物共扩增出152条DNA条带,分子量大小在250-1 600 bp之间,其中有4个引物的扩增结果中检测到了差异性条带,在DNA水平上初步证实了5种花色突变体是由于遗传因素导致的稳定变异。The RAPD analysis of genomic DNA, generated by 10 random primers, 10 nt in every one, discrinainated 5 flower color mutants of novel versicolor mutants in Dahlia pinnata Cav. The results showed that 152 bands were amplified in 10 primers, and each molecular weights range from 250-1 600 bp. Among them particular bands were produced in 4 primers, which suggested primarily in the DNA level that 5 flower color mutants varied due to the genetic factors.

关 键 词：大丽菊 花色突变体 随机扩增多态性DNA 

分 类 号：S682.19[农业科学—观赏园艺] Q944.58[农业科学—园艺学]