滋补肝肾方含药血清对B16黑素细胞活性黑素含量及cAMP水平的影响  

In vitro Study of Drug-contained Serum with Chinese Medicines Nourish Liver and Kidney on B16 Melanocyte Cytotoxicity,Melanin Synthesis and cAMP

作　　者：张蕾[1] 赵京霞[1] 李萍[1] 刘卫红[1] 王莒生[1,2] 

机构地区：[1]首都医科大学附属北京中医医院北京市中医研究所,北京100010 [2]首都医科大学附属北京中医医院,北京100010

出　　处：《辽宁中医杂志》2009年第3期467-469,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助(30572396);北京市自然科学基金资助(7062011)

摘　　要：目的:通过对含滋补肝肾中药大鼠血清对黑素细胞(B16)活性、黑素含量和cAMP水平影响的研究,探讨滋补肝肾方治疗白癜风的机制。方法:体外培养小鼠B16细胞,于对数生长期加不同浓度含滋补肝肾中药的大鼠血清培养后,分别采用MTT法、NaOH法和放免法检测细胞活性、黑素含量和cAMP水平。结果:与空白对照组比较,滋补肝肾方含药血清各剂量组对B16增殖均没有明显促增殖活性;但20%含药血清可明显促进B16合成黑素量(P<0.05),并提高细胞内cAMP水平(P<0.05)。结论:滋补肝肾方可能通过提高黑素细胞cAMP的水平,从而促进B16细胞合成黑素的功能。Objective：To study effects of drug-contained serum with Chinese Medicines nourish liver and kidney on melanocytes （B16） cytotoxicity and melanin synthesis and cAMP;explore the possible roles of nourish liver and kidney decoction in vitiligo treatment. Methods ： Mice B16 cells were cultivated in vitro. Different concentrations rat of drug-contained serum were added to the culture medium during logarithmic growth phase. Cytotoxicity, melanin and cAMP were measured by MTT assay, NaOH assay and radioimmunoassay respectively. Use the rat serum while do not have Chinese Medicine with the same concentrations as controls. Results ：There is no significant difference between the control and the drug-contained serum tested group as to B16 cytotoxicity;as to the level of synthesized melanin and cAMP, there is significant increase （P 〈 0.05 ） in 20% serum drug-contained serum. Conclusion： In vitro study notes that drug-contained serum with herbal decoction of nourish liver and kidney do not have effect on B16 proliferation,while could promote melanin synthesis and level of cAMP.

关 键 词：滋补肝肾方 含药血清 黑素合成 CAMP 

分 类 号：R285.5[医药卫生—中药学]