实验性自身免疫性脑脊髓炎小鼠p38的变化及益肾达络饮的影响  

Effect of Yinshendaluo Decoction on p38 in the Mice of Experimental Autoimmune Encephalomyelitis

作　　者：尚晓玲[1] 高颖[2] 王硕仁[2] 尹岭[3] 张笑明[3] 

机构地区：[1]长春中医药大学基础医学院中医基础教研室,吉林长春130117 [2]北京中医药大学东直门医院中医内科学教育部重点实验室,北京100700 [3]解放军总医院神经信息中心,北京100853

出　　处：《辽宁中医杂志》2009年第3期478-481,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助(30672692)

摘　　要：目的:研究中药复方益肾达络饮对PLP139-151诱导的实验性自身免疫性脑脊髓炎小鼠中枢神经系统内p38的影响,探讨益肾达络饮在神经系统炎性脱髓鞘疾病中的作用机制。方法:采用免疫组化技术,测定各组小鼠中枢神经组织中p38蛋白含量。结果:p38表达正常组与模型组、激素组、中药组比较均有极显著性差异(P值均<0.01);与模型组比较,激素组有显著性差异(P<0.05),中药组有极显著性差异(P<0.01);激素组与中药组比较,有显著性差异(P<0.05)。结论:EAE小鼠p38表达升高,提示EAE病变过程中可能激活p38MAPK信号转导通路。醋酸泼尼松、益肾达络饮均能降低EAE小鼠中枢神经组织内p38的表达,且益肾达络饮优于醋酸泼尼松。益肾达络饮对自身免疫性脑脊髓炎小鼠保护作用可能通过下调p38,抑制p38MAPK活化,从而改善机体免疫紊乱。Objective ：To investigate the effect of Yishendaluo decoction on p38, one kind of mitogen activated protein kinase （MAPK） ,in central nervous system of the mice with PLP139 - 151 - induced experimental autoimmune encephalomyelitis and to explore the mechanism of Yishendaluo decoction on the inflammatory demyelinating diseases in the nervous system. Methods ： Female mice were evenly randomized into normal, model, control and treatment groups. The contents of p38 protein in the four groups above were detected with immunohistochemical method. ResUlts：The contents of p38 in model, control and treatment groups were all significantly higher than those in normal group（P 〈0. 01,0. 01 and 0. 01 respectively）. Compared with model group,the augmentation contents of p38 in central nervous system were reduced significantly in both control and treatment groups （ P 〈 0. 05 and P 〈 0. 01 respectively）, and it was shown statistically significant differences between the two groups above （P 〈 0. 05 ）. Conclusion：The results of present study indicate that Yishendaluo decoction can protect the mouse from the experimental autoimmune encephalomyelitis by attenuating the content of p38,inhibiting the activation of p38 MAPK and improving immunologic derangement.

关 键 词：自身免疫性脑脊髓炎 多发性硬化 益肾达络饮 P38 

分 类 号：R285.5[医药卫生—中药学]