抗坏血酸-钼蓝显色定磷法检测转移因子制剂中核苷酸的含量  

Determination of Nucleotide in Transfer Factor Tablets by a Method of Phosphate Determination Using Ascorbic Acid-Molybdenum Blue Spectrophotometry

作　　者：宋红波[1] 张丽英[1] 

机构地区：[1]黑龙江省药品检验所,15001

出　　处：《黑龙江医药》2009年第2期115-117,共3页Heilongjiang Medicine journal

摘　　要：目的:建立一种测定转移因子制剂中核苷酸含量的方法。方法:采用抗坏血酸-钼蓝显色定磷法,检测核苷酸中有机磷的含量。结果:线性范围为3.015μg/ml-15.075μg/ml,r=0.9999,回收率良好。结论:本方法准确可靠,为转移因子制剂中核苷酸的定量测定提供了一种新的思路。Objective： To establish a new method for the determine of nucleotide in transfer factor tablets. Methos： The content of organophosphate in nucleotide has been as a target, It was determined by molybdenum blue spectrophotometry, with ascorbic acid as the reductant. Resuls： Good recoveries had been got and the linearity range from 3.015μg /ml to 15.075μg /ml. Conclusion： An accurate new method has been established as an alternative of the test of nucleotide in transfer factor tablets.1

关 键 词：钼蓝显色定磷法 转移因子 核苷酸 有机磷 

分 类 号：R927.2[医药卫生—药学]