大珠母贝金属硫蛋白cDNA克隆与序列特征分析  

Analysis on Cloning and Sequence Characteristics of Metallothionein cDNA in Pinctada maxima

作　　者：唐仁生[1,2] 夏建红[2] 王玉梅[2,3] 龚世园[1] 喻达辉[2] 

机构地区：[1]华中农业大学水产学院,湖北武汉430070 [2]中国水产科学研究院南海水产研究所,广东广州510300 [3]上海海洋大学,上海201303

出　　处：《安徽农业科学》2009年第7期2888-2890,2900,共4页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究发展计划863项目(2006AA10A415);广东省科技兴海项目(A200701C02);中央级公益性科研院所基本科研业务费专项资金项目(2007TS07)

摘　　要：[目的]探讨重金属对大珠母贝苗种存活的影响。[方法]对大珠母贝金属硫蛋白基因进行克隆和序列分析。[结果]结果表明,通过构建大珠母贝外套膜全长cDNA文库,首次克隆得到大珠母贝金属硫蛋白(PmMT)全长cDNA序列。该序列全长599 bp,5′UTR(Un-translated region)为75 bp,3′UTR为296 bp,开放阅读框(Open reading frame,ORF)为228 bp,编码75个氨基酸。编码的氨基酸中半胱氨酸含量丰富,达29.3%;赖氨酸和甘氨酸含量也较高,均为9.3%;不含苯丙氨酸、组氨酸、色氨酸和酪氨酸等芳香族氨基酸;含有软体动物等无脊椎动物金属硫蛋白的特征序列。序列特征分析表明,该序列具备金属硫蛋白的典型特征,是金属硫蛋白家族成员。[结论]该研究为海洋贝类金属硫蛋白的进化及功能研究提供基础资料。[Objective]The purpose of this research was to discuss the effect of heavy metal on fingerlings survival of Pinctada maxima. [Method]The metallothionein gene of Pinctada maxima was cloned, sequenced and characterized through construction and screening of the full-length eDNA library with tissue from the mantle of the oyster. [ Result ] The results indicated that the full-length eDNA of PmMT was 599 bp, including a 5＇ untranslated region （UTR） of 75 bp, a 3＇ UTR of 296 bp and an open reading frame （ORF） of 228 bp encoding a polypeptide of 75 amino acids. The contents of cys, lys and gly in the PmMT amino acid sequence were 29.3%, 9.3% and 9.3%, respectively, while no phe, his, trp or tyr was found. PmMT was characterized by the conserved features of invertebrate＇s MTs, indicating that PmMT was a member of the metallothionein protein family. [Conclusion]This research will provide basic information for evolution and function study of marine shellshef metallothionein.

关 键 词：大珠母贝 金属硫蛋白 CDNA文库 

分 类 号：S917[农业科学—水产科学]