用同源重组法将Δ5 Des整合在集胞藻6803中的表达  

Integration and Expression of Δ5 Des Gene in Synechocystis sp.PCC 6803 by Homologous Recombination

作　　者：张柯[1] 张浩玉[1] 姚强[2] 

机构地区：[1]洛阳理工学院环境工程与化学系,河南洛阳471023 [2]河南省洛阳市质量技术监督检验测试中心,河南洛阳471023

出　　处：《安徽农业科学》2009年第7期2891-2893,2949,共4页Journal of Anhui Agricultural Sciences

摘　　要：从枯草芽孢杆菌染色体中扩增出受冷诱导的Δ5Des去饱和酶,构建出可以与集胞藻PCC6803染色体发生同源双交换的质粒,将Km抗性基因::PpsbA1::Δ5脂肪酸去饱和酶基因和对照基因分别整合入PCC6803的染色体上,获得转化子后,对不饱和脂肪酸进行研究。结果发现,30℃条件下脂肪酸成分的含量发生了较大变化,20℃时有新成分的出现,且长链脂肪酸的含量有所上升。Through amplifying the cold-induced A5 desaterase from Bacillus subtilis chromosome, the plasmid that could make the homolngous double-exchange with the chromosome of Synechocystis sp. PCC6803 was constructed. Km resistant gene（PpsbAl ： ：AS desaterase gene） and control gene were integrated into the chromosome of Synechocystis sp. PCC6803. After obtaining the transformant, the unsaturated fatty acids were studied. The results showed that the content of fatty acids had greater changes at 30 ℃. At 20℃, new components was generated and the content of PUFA rose.

关 键 词：Δ5 DES 双交换 EPA 集胞藻PCC6803 

分 类 号：Q78[生物学—分子生物学]