急性ITP患儿外周血中CD_4^+CD_(25)^+Foxp^(3+)Tr细胞、IL-7水平变化及意义  

Changes and significance of CD_4^+CD_(25)^+Foxp^(3+) Tr cells and IL-7 level in peripheral blood of children with acute idiopathic thrombocytopenic purpura

作　　者：金呈强[1] 刘仿[1] 宋丽[1] 肖红[2] 王文涓[1] 

机构地区：[1]广东医学院微生物与免疫教研室,广东湛江524023 [2]广东医学院第一附属医院

出　　处：《山东医药》2009年第11期4-6,共3页Shandong Medical Journal

基　　金：广东省自然科学基金资助项目(06028959)

摘　　要：目的探讨急性特发性血小板减少性紫癜(AITP)的发病机制。方法选择35例AITP患儿(AITP组)和30例健康查体者(对照组),用流式细胞仪检测外周血CD4+CD2+5Foxp3+调节性T(Tr)细胞数量及占CD4+细胞比例,ELISA法检测血浆中IL-7水平。结果AITP组和对照组外周血CD4+CD2+5Foxp3+Tr细胞/CD4+细胞分别为0.11±0.04、0.15±0.02,IL-7分别为(2.32±0.53)、(0.44±0.80)pg/ml,P均<0.05;AITP组外周血IL-7水平与CD4+CD2+5Foxp3+Tr细胞/CD4+细胞呈负相关(r=0.71,P<0.05)。结论CD4+CD2+5Foxp3+Tr细胞数量减少、IL-7水平升高可能在AITP发病中具有重要作用,机制为降低有效免疫抑制作用,导致自身反应性T细胞激活增多、凋亡减少,促进血小板破坏。Objective To investigate the pathogenesis of acute idiopathic thrombocytopenic purpura（AITP）. Methods 35 cases with AITP （ AITP group） and 30 healthy cases （ control group） were selected, the quantity of the CD4^＋CD25^＋Foxp3^＋ Tr cells and proportion of CD4^＋CD25^＋Foxp3^＋Tr cells/CD4^＋ cells were detected by flow cytometry, and the level of IL-7 were detected by ELISA. Results The proportion of CD4^＋CD25^＋Foxp3^＋ Tr cells/CD4^＋ cells in AITP group and control groupwere 2.96% ± 1.05% and 5.37% ± 0.69% （ P 〈 0.05 ） , while the level of IL-7 were （ 2.32 ±0. 53） and （0.44 ± 0.80）pg/ml（P 〈0.05 ）. The level of IL-7 in AITP group was negatively correlated with the proportion of the CD4^＋CD25^＋Foxp3^＋ Tr cells / CD4^＋ cells （ r = 0.71,P 〈 0.05）. Conclusion The quantity reduction of CD4^＋CD25^＋Foxp3^＋Tr ceils and up-regulation of IL-7 may play important role in the pathogenesis of AITP ,the mechanism maybe the the function weakness of immunosuppression.

关 键 词：紫癜 血小板减少性 特发性 CD4+CD25+Foxp3+调节性T细胞 白细胞介素-7 

分 类 号：R554.6[医药卫生—血液循环系统疾病]