自制愈障汤对再生障碍性贫血大鼠骨髓造血细胞线粒体形态及跨膜电位的影响  

Effect of Yuzhang Tang on the mitochondria morphology and transmembrane potential of bone marrow cells in mice with aplastic anemia

作　　者：陈慧莉[1,2] 李建华[1] 郝桂兰[1] 邵明霞[1] 李来传[1] 王树庆[1] 杨兆禄[1] 

机构地区：[1]潍坊医学院附属医院,山东潍坊261031 [2]北京航空工业中心医院

出　　处：《山东医药》2009年第11期7-9,共3页Shandong Medical Journal

基　　金：山东省中医管理局计划课题资助项目(2003年第126号)

摘　　要：目的进一步探讨自制愈障汤治疗再生障碍性贫血(AA)的作用机制。方法将80只Wistar大鼠随机分为四组各20只,对照组行铅砖屏蔽下X线假照射;余三组均参照文献制备AA模型。自照射当天开始,模型组和对照组均胃饲生理盐水2ml/d,中药组和西药组分别胃饲愈障汤和环孢菌素A(CsA)各2ml/d。于实验第10、16天光镜计数外周血细胞及骨髓有核细胞;透射电镜观察骨髓造血细胞线粒体超微结构,流式细胞术检测线粒体跨膜电位。结果①中药组外周血、骨髓有核细胞计数均显著高于模型组,淋巴细胞系Rh123-PI-细胞百分率显著低于西药组;对照组骨髓造血细胞线粒体结构无明显异常,模型组大部分线粒体嵴断裂、模糊、嵴间腔消失;中药组和西药组细胞线粒体结构接近正常。②与模型组比较,实验第10天中药组中性粒细胞亚群Rh123-PI-细胞百分率显著降低(P<0.01)。结论愈障汤能够明显改善AA大鼠粒细胞亚群线粒体结构,抑制跨膜电位下降及细胞凋亡,此可能为其治疗AA的作用机制之一。Objective To investigate the treatment mechanism of Yuzhang Tang on aplastic anemia （AA）. Methods 80 Wistar mice were randomly divided into four groups with 20 in each ,except the control group,the other three groups were made models of AA. The control group and the model group were given normal saline by gastrogavage, while the Chinese medicine group and the western medicine group were given Yuzhang Tang and CsA respectively. On the 10th day and 16th day, peripheral blood cell （PBC） and nucleated bone marrow cell（NBMC） were calculated by optical microscope,mitochondrial uhrastructure and transmenahrane potential of myeloid element were observed by transmission electron microscopeand flow cytometry. Results ①PBC and NBMC in the Chinese medicine group was higher than that in the model group , while the percentages of Rh123-PI- cells of neutrophile granulocyte subgroup was lower than that in the western medicine group. Mitochondrial ultrastructure in the model group showed mitochondrial crista rupture, faintness and disappearance, while which in the other three group was almost normal. ②Compaxcd with the model group, the percentages of Rh123-PI- cells of neutrophile granulocyte subgroup On the lOth day in Chinese medicine group decreased significantly（ P 〈0. 01 ）. Conclusion Yuzhang Tang can improve the mitochondrial ultrastructure significantly , suppress the down-regulation of transmembrane potential and apoptosis, which may be one of its treatment mechanism on AA.

关 键 词：贫血 再生障碍性 愈障汤 骨髓细胞 线粒体 跨膜电位 细胞凋亡 

分 类 号：R556.5[医药卫生—血液循环系统疾病]