CD59特异位点短肽封条对T细胞封闭作用  

THE BLOCKING EFFECT OF PEPTIDE SEALS SPECIFIC FOR CD59 ON T CELLS

作　　者：孙庆燕[1] 高美华[1] 

机构地区：[1]青岛大学医学院免疫学教研室,山东青岛266021

出　　处：《青岛大学医学院学报》2009年第2期95-96,100,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金资助项目(3170893)

摘　　要：目的探讨含有CD59特异位点的短肽封条对T细胞的封闭作用及其可能机制。方法建立HeLa肿瘤小鼠模型,眼球取血分离血清测定补体C3、C4,分离培养鼠胸腺T淋巴细胞、分别加入CD59-mAb、CD59特异位点短肽封条作用48h后,MTT法检测T细胞增殖情况。结果肿瘤小鼠补体C3、C4含量明显降低(t=30.970、16.789,P<0.01);CD59-mAb对T细胞增殖起促进作用,而含有CD59特异位点的短肽封条能抑制T细胞增殖,两者的作用与正常对照相比差异有显著性(t=5.428、4.726,P<0.01)。结论CD59参与T细胞信号转导而与补体抑制作用无关,含有CD59特异位点的短肽封条对T细胞起封闭作用。Objective To study the blocking effect of peptide seals specific for CD59 on T ceils and their possible mechanisms. Methods A mouse tumor model was established. Blood samples were obtained from the eyes, and serum C3 and C4 determined. T cells were isolated from thymus and cultured with CD59-mAb and peptide seals specific for CD59 for 48 hours. The proliferation of T ceils was tested by MTT. Results The serum levels of C3 and C4 reduced significantly （t = 30. 970,16. 789; P〈0.01）. CD59-mAb promoted the proliferation of T cells, but the peptide seals specific for CD59 inhibited T cell proliferation. There were significant differences between the experiment and control groups in both CD59-mAb and peptide seal experiments （t= 5. 428,4. 726 ;P〈0.01）. Conclusion CD59 is involved in the signal transduction of T ceils, but has no relationship with complement inhibition. The peptide seals specific to CD59 can block the CD59 of T cells.

关 键 词：抗原 CD59 短肽封条 肿瘤 T淋巴细胞 细胞增殖 

分 类 号：R392.12[医药卫生—免疫学]