氯胺酮抗士的宁惊厥作用的研究及ED_(50)的测定  

Effects of ketamine against strychnine-induced convulsions and the determination of its ED_(50) in mice

作　　者：鲍捷[1] 陈湉[1] 王莉[1] 孙煦[1] 黄腾[1] 孟晶[2] 

机构地区：[1]徐州医学院麻醉学院,2005级4班江苏徐州221004 [2]徐州医学院麻醉药理学教研室

出　　处：《徐州医学院学报》2009年第3期171-172,共2页Acta Academiae Medicinae Xuzhou

基　　金：江苏省高校自然科学基础研究项目(07KJD310219);徐州医学院课题(07KJ029)

摘　　要：目的测定氯胺酮抗硝酸士的宁小鼠惊厥的ED50。方法将昆明种小鼠随机分为6组，每组10只，分别腹腔注射生理盐水（NS）或氯胺酮24．5、35．0、50．0、71．4、102．0mg／kg，5min后分别腹腔注射硝酸士的宁1．5mg／kg，观察小鼠的惊厥发生率、潜伏期、强直持续期和死亡率，用序贯法、点斜法测定氯胺酮抗硝酸士的宁小鼠惊厥的ED50。结果与NS组比较，K50.0组、K71.4组和K102.0组惊厥发生率均降低（P〈0．05或P〈0．01），如K35.0、K50.0、K71.4和K102.0组小鼠死亡率均降低（P〈0．05或P〈0．01），各组惊厥潜伏期均延长（P〈0．05或P〈0．01），且呈剂量依赖性（r=0．9740，P〈0．01）。氯胺酮抗士的宁惊厥的点斜法ED50的95％可信区间是44．8～64．3mg／kg。序贯法ED50的95％可信区间是39．3—74．8mg／kg。结论氯胺酮的抗士的宁小鼠惊厥ED50的95％可信区间分别为44．8～64．3mg／kg和39．3—74．8mg／kg，其机制可能与甘氨酸受体有关。Objective To determine the effects of ketamine and its 50% effective dose （ ED50 ） during its therapeutic use against strychnine - induced convulsions in mice. Methods Kunming mice randomly divided into six groups （n =10） were given intraperitoneal injection of saline 0. 1 ml/10 g or doses of ketamine （24.5, 35.0, 50. 0, 71.4 and 102.0 mg/kg）, respectively. 5 minutes later, 0.1 ml/kg of 1.5 mg/kg strychnine nitrate was administered i.p. to observe the incidence of seizures in mice, latency, duration of tonic state and mortality, and to determine the anticonvulsant ED50 of ketamine against strychnine - induced convulsions in mice using sequential method and spot - slope method. Results Compared with the NS group, the incidences of seizures decreased in K50.0, K71.4 and K102.0 groups （ P 〈 0.05, P 〈 0.01 ） ; the mortalities significantly decreased in K35. 0, K50 .0, K71.4 and K102.0 groups （P 〈 0.05, P 〈 0.01 ）, the sei- zure latencies were prolonged in all groups （ P 〈 0.05, P 〈 0.01 ） ; in a dose - dependent manner each group can be shortened seizure latency （P 〈 0.05, P 〈 0.01 ）, and a dose - dependent manner （ r = 0. 9740, P 〈 0.01 ）. The 95% authentic range of ketamine EDs0 against strychnine convulsion was 44.8 - 64.3 mg/kg by the grouping method and 39.3 - 74.8 mg/kg by the sequential method. Conclusion The anticonvulsant mechanisms of ketamine may, be associated with glycin receptor.

关 键 词：氯胺酮 士的宁 惊厥 小鼠 半数有效量 

分 类 号：R971.1[医药卫生—药品]