不同海拔青海云杉与祁连圆柏叶片抗氧化酶活性的研究  

Study on antioxidase activities of Picea crassifolia and Sabina przewalskii at different altitude gradients

作　　者：陈晓莉[1] 张鹏[1] 张涛[1] 陈年来[1,2] 陈拓[2] 

机构地区：[1]甘肃农业大学资源与环境学院,甘肃兰州730070 [2]中国科学院寒区旱区环境与工程研究所,甘肃兰州730000

出　　处：《甘肃农业大学学报》2009年第1期118-122,共5页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(30670319,30770342)

摘　　要：以寺大隆林区连续海拔梯度(2 765 m^3 565 m)上青海云杉和祁连圆柏为材料,通过测定叶片中抗氧化保护酶活性的变化,探讨常绿木本植物抗氧化酶系统适应高山极端环境的机制.结果表明:随着海拔升高,丙二醛(MDA)、可溶性蛋白、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等几种物质在青海云杉(除SOD活性)和祁连圆柏叶片中含量均虽增加趋势;相同海拔梯度上,青海云杉的MDA含量高于祁连圆柏,而可溶性蛋白含量低于祁连圆柏,说明2树种都能适应低温、冰冻、强辐射等环境胁迫,但祁连圆柏的抗低温胁迫和抗辐射能力高于青海云杉.Taking the leaves of Picea crassifolia and Sabina przewalskii at different altitude gradients （2 765 m-3 565 m） in Sidalong forests as the experimental materials, the antioxidase activities of the leaves were determined, and based on the determination results, the mechanism of how evergreen xylophyte antioxidase system adapting to the mountain extreme environment was discussed. The results showed as follows： with the increasing altitude, contents of MDA, soluble protein, superoxide dismutase （SOD）, the activities of peroxidase （POD） and catalase （CAT） were all increased in both Picea crassifolia and Sabiina przewalskii;when at the same altitude gradient, the content of MDA was higher in Picea crassifolia than that in Sabina przewalskii, but the content of soluble protein was opposite. These indicated that both trees could adapt to low temperature, frozen and strong radiation stresses, but Sabina przewalskii had the higher ability of resisting low temperature and strong radiation stress.

关 键 词：海拔梯度 抗氧化酶 适应性 青海云杉 祁连圆柏 

分 类 号：S718.43[农业科学—林学]