凝血因子ⅩⅢB亚单位遗传多态性在中国七个群体的分布  

GENETIC POLYMORPHISM OF FⅩⅢB SUBUNIT IN SEVEN CHINESE POPULATIONS

作　　者：陈雷铃[1] 侯一平[1] 金泽明[1] 吴谨[1] 李英碧[1] 苟清[1] 徐萍蓉 

机构地区：[1]华西医科大学法医学系

出　　处：《中华医学遗传学杂志》1998年第1期13-16,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金

摘　　要：目的评估凝血因子ⅩⅢＢ亚单位（ＦⅩⅢＢ）遗传多态性在中国群体中的分布情况。方法建立对ＦⅩⅢＢ遗传表型进行分型的等电聚焦及免疫印迹分型方法，通过此方法调查了中国７个群体（共８１９个体）ＦⅩⅢＢ遗传多态性。结果群体调查显示在这七个群体中存在３个常见等位基因，频率以ＦⅩⅢＢ＊３为最高，ＦⅩⅢＢ＊１次之，ＦⅩⅢＢ＊２最低，各等位基因频率均达到多态性水平。另外尚在一些群体中发现了一种稀有型等位基因。７个群体间基因型构成无显著性差异（Ｐ＞０．０５）。这样的分布模式反映了ＦⅩⅢＢ基因频率在中国人群中的分布概况，即中国人群中以ＦⅩⅢＢ＊３基因频率为主，以ＦⅩⅢＢ＊２基因频率最低。进一步的遗传距离与系统树分析提示ＦⅩⅢＢ是一个很好的人类群体遗传标记。结论ＦⅩⅢＢ是一个有较好应用价值的群体遗传学及法医学血液遗传学遗传标记。Objective To understand the distribution of allele frequencies of blood factor ⅩⅢB subunit (FⅩⅢB) in Chinese populations and evaluate the genetic polymorphism of FⅩⅢB for the purposes of population genetics and forensic haemogenetics. Methods The genetic polymorphism of FⅩⅢB subunit in seven Chinese populations was investigated. Isoelectrofocusing technique on polyoacrylamid gels followed by immunoblotting was used to determine the phenotype of individuals in each population sample. Results There were three common alleles in all the seven Chinese populations.The frequency of FⅩⅢB *3 was the highest,that of FⅩⅢB*2 was the lowest,and the one of FⅩⅢB*1 was at the middle.All of them reached to the polymorphism's level.A rare variant allele was also found in some Chinese populations.Comparison of the constituents of FⅩⅢB phenotypes in the seven populations showed that there was no significant difference ( P >0 05).The distribution of the allele frequencies in these populations reflected most likely the mode of the distribution in all Chinese populations.The phylogenetic tree and genetic distance,based on the allele frequencies of FⅩⅢB differentiated the populations in the world into the main ethnic groups as what other authors reported.Conclusion FⅩⅢB is a useful genetic marker for population genetics and forensic haemogenetics.

关 键 词：凝血因子 FⅩⅢB 亚单位 遗传多态性 中国 

分 类 号：Q346.5[生物学—遗传学] Q987