ELISA与FAVN方法检测犬狂犬病抗体的比较  

A comparative detection of the anti-rabies antibodies by ELISA and FAVN

作　　者：孙招金[1] 薛素强[2] 刘晓慧[1] 郭霄峰[1] 郭慧霞[1] 

机构地区：[1]华南农业大学兽医学院微生物教研室,广东广州510642 [2]华南农业大学生物药品有限公司,广东增城511300

出　　处：《中国预防兽医学报》2009年第3期204-206,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家高技术研究发展计划(2006AA10A204);农业公益性行业专项(200803014)

摘　　要：比较酶联免疫吸附试验(ELISA)与细胞培养病毒中和试验(FAVN)检测狂犬病疫苗免疫后血清抗体。将40只犬免疫兽用狂犬病疫苗后,第14d静脉采血分离血清,分别采用ELISA法和国际贸易指定试验FAVN法检测血清样品,试验犬免疫第21d攻击狂犬病毒BD06株。结果表明,两种方法检测结果差异不显著(p≥0.05,p=0.19),FAVN、ELISA法检测狂犬病抗体与攻毒试验结果的阳性符合率均为100%。This study aimed to compare the effeciveness of ELISA and FAVA in detection of antibody against rabies vaccine. Healthy dogs were inoculated with rabies vaccine and antibody titre was measured by ELISA and FAVN method from the 14th day. The immunized dogs were challenged with rabies virus BD06 strain. No significant different between ELISA and FAVN was found （p≥0.05, p=0.19）. The positive coincidence between the ELISA and Rabies virus challenge of vaccinated dogs was 100 %.

关 键 词：狂犬病抗体 ELISA FAVN 

分 类 号：S858.292[农业科学—临床兽医学]