四价鸡球虫苗的免疫效果研究  

The protective efficacy of the live vaccine against coccidiosis

作　　者：闫鸿斌[1] 贾万忠[1] 田广孚[1] 李万坤[1] 孟庆玲[1] 郭爱疆[1] 才学鹏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室/农业部兽医公共卫生重点实验室,甘肃兰州730046

出　　处：《中国预防兽医学报》2009年第3期236-240,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家高技术研究发展计划"863计划"(2006AA10A207);国家科技支撑计划(2007BAD40B04);国家自然科学基金项目(30872369)

摘　　要：在实验条件下观察自主研制的四价鸡球虫苗(LCV)对平养鸡的安全性和免疫保护效果。试验设空白对照组(NC)、攻虫对照组(PC)和免疫组(VC)3个试验组,每组30只鸡。试验结果:空白对照组、攻虫对照组和免疫组的相对增重率依次为100%、84.77%和94.97%;免疫后(免疫后攻虫前)排卵囊高峰期间(免疫后5d～10d),免疫组平均OPG(即每克粪便中的卵囊数)为2.70×104,攻虫后排卵囊高峰期间(攻虫后6d～13d)攻虫对照组组平均OPG为2.32×106,高于免疫组(2.69×105)(p<0.01);攻虫后各组死亡率依次为0、10%和0;3个组的ACI(抗球虫指数)分别为200.0、154.77和184.97,免疫组试验效果最佳。结果表明,LCV安全性高、免疫保护效果理想,接种该疫苗后的鸡对较大剂量攻虫具有较强的抵抗力。The protective efficacy of a vaccine against coccidiosis was examined in Lingnanhuang female broilers kept in floor-pens. Three trail groups, including negative group （NC）, positive group （PC） and vaccinated group （VC） was tested, and each group comprised 30 broilers. The results showed that the average relative ratio of weight gain of 3 groups were 100 %, 84.77 % and 94.97 %, respectively. The OPG of PC group （2.32× 10^6） was significantly higher （p〈0.01） than vaccinated group （2.69×10^5） after challenge infection. The mortality rate of 3 groups were 0, 10 % and 0, respectively. Furthermore, higher ACI were detected in NC （200） and VC birds （184.97）, which were significantly higher than that of PC birds （154.77）.

关 键 词：鸡 球虫 疫苗 LCV 免疫 

分 类 号：S852.723[农业科学—基础兽医学]