膀胱移行上皮细胞癌中穿通支原体的检出及意义  

Detection of Mycoplasma penetrans in transitional cell carcinoma of bladder

作　　者：朱燕英[1] 余志贤[2] 周丽萍[3] 

机构地区：[1]温州医学院附属第一医院实验诊断中心,温州325000 [2]温州医学院附属第一医院泌尿外科 [3]温州医学院检验学院

出　　处：《中国人兽共患病学报》2009年第3期238-240,共3页Chinese Journal of Zoonoses

基　　金：温州市科技局资助项目(Y20070059)

摘　　要：目的探讨穿通支原体(Mycoplasma penetrans,Mpe)在膀胱移行上皮细胞癌患者血液和肿瘤组织中的分离检出及意义。方法将55例膀胱移行上皮细胞癌患者的膀胱组织标本、血液标本分别接种于改良SP-4液体培养基中进行支原体分离培养,可疑阳性菌株用PCR予以证实。结果55例患者的Mpe累计分离率为41.8%(23/55),对照组的分离率为11.1%(3/27),两组比较有统计学意义(P<0.05)。将患者按UICC-TNM标准进行分期,浸润癌(T2~T4)组Mpe分离率为69.0%(9/13),非浸润癌(Ta^T1)组为33.0%(14/42),两组比较差异也有统计学意义(P<0.05)。结论膀胱移行上皮细胞癌患者的膀胱组织、血液中Mpe分离阳性率高,且浸润性膀胱癌组较非浸润癌组高,提示Mpe感染与膀胱移行上皮细胞癌的发生有一定关联,与肿瘤是否浸润也存在一定关系。Mycoplasma penetrans was isolated from blood and tissue specimens of 55 patients with transitional cell carcinoma of bladder and 27 cases of controls by means of cultivation of the organisms on modified SP-4 liquid media. Then the posi-tive isolates were confirmed by nested polymerase chain reaction （nPCR）. It was found that the positive detection rate for M. penetrans was 41.8% （23/55）in patients of transitional cell carcinoma of bladder, while it was only 11.1%（3/27）in the control group. The difference of M. penetrans isolation between superficial bladder cancer （Ta-T1） and invasive bladder cancer （T2-T4） was statistically evident（P〈0.05）. It is concluded that the higher isolation rate of M. penetrans in blood and tissues of patients with transitional cell carcinoma of bladder may have. some association with the development of transitional cell carcinoma of bladder.

关 键 词：穿通支原体 膀胱移行上皮细胞癌 癌 

分 类 号：R375[医药卫生—病原生物学]