基于组织工程皮肤的化学品光毒性检测  

Phototoxicity test of chemicals based on tissue engineered skin

作　　者：邹运动[1] 曹玉萍[2] 郑洪艳[1] 杨晓冉[1] 李铭[1] 任冬梅[1] 董益阳[1] 

机构地区：[1]中国检验检疫科学研究院,北京市100123 [2]中国医学科学院皮肤病研究所,江苏省南京市210042

出　　处：《中国组织工程研究与临床康复》2009年第11期2147-2150,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：中国检验检疫科学研究院基本科研业务费专项基金(2007JK017)~~

摘　　要：组织工程皮肤是指应用生命科学与工程学的原理与技术,研究并开发出的用于修复、维护、促进人体皮肤损伤后功能和形态的皮肤替代物。它有2个主要的应用方向:体内主要运用于各种皮肤创伤的治疗,体外主要作为皮肤模型检测化学品的安全性。化学品的安全性越来越受到人们的重视,光毒性的检测是其中的一项重要内容。鉴于对动物福利和动物保护的日趋重视,近些年出现了多种体外替代检测方法。文章重点综述了基于组织工程皮肤的化学品光毒性替代检测情况,具体涉及组织工程皮肤的介绍、光毒性概念和发生机制、光毒性替代检测、参考化合物选择和结果的判定等方面内容。随着组织工程理论和技术的发展,基于组织工程皮肤的化学品安全性检测的敏感度、特异性和精确度会进一步提高。Tissue engineered skin refers to developing biological substitutes aimed at the replacement, repair, maintenance, and/or enhancement of skin function by using the principles of engineering and life sciences. It mainly has two application fields： improving wound healing in vivo and to test the chemical safety in vitro. Nowadays, the public pays more attention on chemical safety, in which phototoxicity is one of the most important parts. In consideration of animal welfare and animal protection, various types of in vitro tests were developed over decades. The aim of the present review is to show phototoxicity testing based on tissue engineered skin, contains brief introduction of tissue engineered skin, definition and mechanism of phototoxicity, in vitro phototoxicity test, selection of reference chemicals and endpoints. With the development of theory and technology, tissue engineering will greatly improve the sensitivity, specificity and accuracy of chemical safety test based on tissue engineered skin.

关 键 词：组织工程皮肤 化学品安全 替代方法 光毒性 

分 类 号：R318[医药卫生—生物医学工程]