成骨细胞特异性钙黏蛋白基因转染对人骨髓基质干细胞成骨分化影响的研究  

Effect of OB-cadherin gene transfection on osteogenic differentiation of human mesenchymal stem cells in vitro

作　　者：向强[1] 邓聪颖[1] 郑文杰[1] 郭国宁[1] 张远[1] 张超[1] 周跃[1] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400037

出　　处：《中华创伤骨科杂志》2009年第3期259-262,共4页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金资助项目（30700892,30572166）;重庆市自然科学基金资助项目（CSTC,2007BB5058）

摘　　要：目的观测成骨细胞特异性钙黏蛋白（Cad—Ⅱ）基因转染对人骨髓基质干细胞（hBMSCs）成骨分化的影响。方法把脂质体介导的Cad-Ⅱ cDNA转染体外分离培养的hBMSCs，检测Cad-Ⅱ蛋白表达变化，并对比观测转染组和单纯成骨诱导组在培养3，7、14、21d时，hBMSCs碱性磷酸酶（ALP）和骨钙素的表达变化。结果转染组和成骨诱导组3d后开始有ALP的阳性染色，呈棕黑色，7d开始有骨钙素的阳性染色并随培养时间延长逐渐增多，但各时间点转染组ALP浓度（P=0．008）和骨钙素染色阳性数（P=0．023）均显著高于单纯成骨诱导组。转染组和成骨诱导组从14d后开始有红色的阳性染色矿化结节出现，结节数目随时间推移而增多。结论Cad—Ⅱ基因转染可促进hBMSCs向成骨细胞的分化。Objective To study the effects of Cad- Ⅱgene on osteogenic differentiation of human mesenchymal stem cells in vitro. Methods The human marrow mesenchymal stem cells （hBMSCs） were isolated and cultured in vitro before they were divided into 2 groups. In the transfeetion group, the hBMSCs were transfeeted with Cad- Ⅱgene; in the simple osteogenie inducement group, they were cultured in the condition medium. Then the expressions of Cad- Ⅱ protein were determined and the alkaline phosphatase （ALP） activities and the expressions of osteocalcin were measured at 3, 7, 14 and 21 days for comparison between the 2 groups. Results The ALP activity and positive expression of osteocalcin were regulated significantly higher in the transfection group than in the simple osteogenie inducement group at different times （ P 〈 0. 05）. The mineralized nodes began to appear at 14 days in the 2 groups and increased with time. Conclusion Cad- Ⅱ gene transfection can promote differentiation of hBMSCs into the osteoblasts.

关 键 词：钙黏着糖蛋白类 Cad-Ⅱ 基质干细胞 成骨分化 支架 

分 类 号：R686[医药卫生—骨科学]