血液灌流对急性草乌中毒兔血浆毒性成分及组织病理学的影响  

Effects of hemoperfusion on toxic ingredients in plasma and histopathology in acute rabbits with acute intoxication of Radix Aconiti Kusmezoffii

作　　者：邱俏檬[1] 刘刚[1] 卢中秋[1] 王志翊[1] 梁欢[1] 

机构地区：[1]温州医学院附属第一医院急诊科,浙江省温州325000

出　　处：《中华急诊医学杂志》2009年第3期247-251,共5页Chinese Journal of Emergency Medicine

基　　金：基金项目：浙江省医学扶植重点建设学科计划资助（07-FU4）;浙江省中医药管理局资助项目（2006C094）

摘　　要：目的探讨血液灌流对急性草乌中毒兔血浆毒性成分和组织病理学的影响。方法雄性日本大耳白兔16只，随机分成中毒组（AP组，n=8）和血液灌流治疗组（AH组，n=8）。两组用草乌酒1mL／kg灌胃，制作急性中毒模型（以1h内出现心律失常为制模成功标准），AH组用活性碳血液灌流2h。光镜下观察两组兔脑、心肌、肝组织病理改变。分别比较两组相同时间点血浆中乌头碱、新乌头碱、次乌头碱浓度。两样本均数比较采用成组t检验。结果AP组兔脑组织、心肌组织、肝组织充血水肿明显，AH组兔的肝脏组织轻度充血，脑组织、心肌组织未见明显改变；两组染毒后1h内血浆乌头碱、新乌头碱、次乌头碱浓度比较，差异无统计学意义（P〉0．05）。AH组染毒后2h，3h血浆乌头碱、新乌头碱、次乌头碱浓度分别为[（2．11±1．08），（2．02±1．46）]，[（39．70±9．31），（19．71±16．06）]，[（1．70±0．71），（2．12±1．33）]ng／mL，均明显低于AP组，差异有统计学意义（P〈0．05）。结论急性草乌中毒兔血液灌流治疗后血浆中草乌成分浓度明显降低，器官组织病理改变减轻，血液灌流是兔急性草乌中毒的有效治疗手段。Objective To explore effectS of hemoperfusion on toxic ingredients in plasma of rabbits with acute intoxication of Radix Aeoniti Knsmezoffii Monkshood. Method Sixteen male Japanese Giant Ear Rabbits were randomly divided into acute poisoning （AP） group and acute poisoning ＋ hemoperfnsion （AH） group （ 8 animals in each group）. Acute poisoning models were established in rabbits of both groups with intragastrie administration of Radix Aeoniti Kusmezoffii Monkshood liquid in dose of 1 mL/kg in order to produce arrhythm which occurred within one hour after intragastric administration was regarded as the criteria of successful animal model, and then hemoperfusion with active carbon was performed for 2 hours in AH group. The pathological changes of brain, myocardium and hepatic tissues were observed. The plasma concentrations of toxicants including mesaeonitine, aeonitine and hypaconitine were measured by using HPLC-MS at 1 h, 2 h, 3 h, and 6 h after poisoning. Student＇ s T test was used to identify the significance. Results The brain, myocardium and hepatic tissues of the rabbits in AP group showed hyperemia and edema which were attenuated after hemoperfusion. The plasma concentrations of mesaconitine, aconitine and hypaeonitine revealed no significant differences between AP group and AH group within one hour after poisoning （ P 〉 0.05）, while at 2 h and 3 h after poisoning, the plasma concentrations of mesaconitine were （2.11±1.08） ng/mL, （ 2.02 ± 1.46 ） ng/mL, respectively, aconitine （39.70 ± 9.31 ） ng/mL, （19.71 ± 16.06）ng/mL, respectively, and hypaconitine（1.70±0.71）ng/mL, （2.12±1.33） ng/mL, respectively in AH group, and they were significantly lower than those in AP group （ P 〈 0.05）. Conclusions The the plasma concentrations of mesaconitine, aconltine and hypaeonitine were lower and the histopathologlcal changes were attenuated after hemoperfusion. Hemopeffusion is a good intervention for acute intoxication of Radix Aconiti Knsmezoffii Monkshood.

关 键 词：草乌 中毒 血液灌流 组织病理学 高效液相-质谱分析 

分 类 号：R595.4[医药卫生—内科学] R739.5[医药卫生—临床医学]