碳纤维增强聚醚醚酮与钛合金血液相容性的比较  

Comparison of blood compatibility between carbon fibers reinforced polyetheretherketone and titanium alloy

作　　者：刘军[1] 邢宏健[1] 吴敏飞[1] 朱庆三[1] 杨小玉[1] 

机构地区：[1]吉林大学第三临床医学院(中日联谊医院)骨科,吉林省长春市130033

出　　处：《中国组织工程研究与临床康复》2009年第8期1455-1458,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助(10372034)~~

摘　　要：背景:碳纤维增强聚醚醚酮是聚醚醚酮的改进复合材料,改善了纯聚醚醚酮强度不足的缺点,扩大了其在各方面的使用范围。但若要成为应用于人体内的医用材料,必须具有良好的血液相容性。目的:比较碳纤维增强聚醚醚酮和钛合金对人血细胞的影响,判断碳纤维增强聚醚醚酮是否具有良好的血液相容性。设计、时间及地点:对比观察实验,于2008-06/08在吉林大学第三临床医学院中心实验室完成。材料:取实心钛合金股骨干髓内针1根及含20%碳纤维的碳纤维增强聚醚醚酮样条若干,机加工成棒形试样(长5mm,直径1mm)各20个。方法:在相同条件下,将钛合金和碳纤维增强聚醚醚酮2种材质的试件分别放置于新鲜人血中,通过观察溶血率,血小板激活程度以及白细胞激活程度判断材料的血液相容性。主要观察指标:应用游离血红蛋白直接测定法测量材料溶血率;流式细胞术测量血小板CD62p、CD63含量和白细胞表面黏附分子CD11b/CD18表达。结果:2种材料溶血率,血小板CD62p含量、CD63含量、白细胞表面黏附分子CD11b/CD18表达水平差异无显著性意义(P>0.05)。结论:碳纤维增强聚醚醚酮和钛合金对人血红细胞、白细胞和血小板无显著影响,均具有良好的血液相容性。BACKGROUND： Carbon fibers reinforced polyetheretherketone （CFR/PEEK） is a modified compound material of poly ether-ether-ketone （PEEK）, improving weak intensity of pure PEEK and enlarging its serviceable range. However, excellent blood compatibility is necessary for clinical application. OBJECTIVE： To compare the effects of CFR/PEEK and titanium a/toy on human hemocytes, and determine whether CFR/PEEK has good blood compatibility. DESIGN, TIME AND SETTING： A contrast observational study was performed at the Central Laboratory of the Third Clinical Medical College of Jilin University between June and August 2008. MATERIALS： Machine-processed 40 stick samples （5 mm in length and 1 mm in diameter） were made using one titanium alloy femur stem nail and several CFR/PEEK samples （containing 20% carbon fibre） as raw materials. METHODS： Under the same conditions, the samples of titanium alloy and CFR/PEEK were placed in the fresh human blood so as to determine blood compatibility via haemolysis rate, platelet activation level, and leukocyte activation level. MAIN OUTCOME MEASURES： Haemolysis rate was measured with direct free hemoglobin assay; contents of CD62p and CD63 as well as CD11b/CD18 expression were measured using flow cytometry. RESULTS： There were no significant differences in heamolysis rate, contents of CD62p and CD63, and CD1 lb/CD18 expression between CFR/PEEK and titanium alloy （P 〉 0.05）. CONCLUSION： Both CFR/PEEK and titanium alloy have no significant effects on human erythrocytes, leucocytes, and platelets, suggesting that they have a satisfactory blood compatibility.

关 键 词：碳纤维增强聚醚醚酮 钛合金 血液相容性 

分 类 号：R318[医药卫生—生物医学工程]