骨与生物材料界面的透射电镜与体内杂交方法分析  

An analysis of bone-biomaterial interface using transmission electron microscopy and in vivo hybridization

作　　者：辛风[1] 曾晖[1] 李继云[1] 张波[1] 

机构地区：[1]北京大学深圳医院骨科,深圳市518036

出　　处：《中国组织工程研究与临床康复》2009年第8期1553-1556,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：生物活性材料如生物玻璃、羟基磷灰石、含有羟基磷灰石-硅灰石的玻璃陶瓷等都可与骨组织结合在一起。其是否直接与骨接触,与骨结合的速度是否存在差别,同时材料本身是否有促进骨形成的能力等诸多方面的问题还有待阐明。通过透过电子显微镜来观察生物材料与骨的界面,研究磷灰石层与材料的生物活性之间的关系,应用在体杂交方法探讨材料周围细胞中mRNA的表达以及其对材料的影响,提示玻璃陶瓷要比羟基磷灰石的生物活性高,与骨组织更加容易地结合在一起。The bioactive materials, such as biological glass, hydroxyl apatite, glass ceramics which contains the hydroxyl apatite and wollastonite, are possible to unify with the bone tissue together. However, many questions like whether the materials contact and unify directly with the bone, whether the speeds of unification have the differences, and whether materials themselves simultaneously have abilities to promote the bone formation are not clear and wait for the exposition. The researches have been made to observe the contact surface between biological material and bone, as well as the relationship among the apatite level and the bioactivity of materials through the transmission electron microscopy. In addition, in vivo hybridization has been used to discuss the mRNA expression and its influence in the cell around materials. The conclusion is that the bioactivity of glass ceramics is higher than that of hydroxyl apatite, with the easy unification with bone tissue.

关 键 词：生物材料 羟基磷灰石 骨基质蛋白 生物活性 

分 类 号：R318[医药卫生—生物医学工程]