钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用  

Regulation effect of calcium and salicylic acid on defense enzyme activities in tomato leaves under sub-high temperature stress

作　　者：李天来[1] 李淼[1] 孙周平[1] 

机构地区：[1]沈阳农业大学园艺学院辽宁省设施园艺重点实验室,沈阳110161

出　　处：《应用生态学报》2009年第3期586-590,共5页Chinese Journal of Applied Ecology

基　　金：国家"十一五"科技支撑计划项目(2006BAD07B04-3)资助

摘　　要：为了明确Ca2+和水杨酸对番茄耐亚高温特性的调控作用,在番茄第1花序第1花开花当天进行昼间亚高温(35℃)胁迫处理,并在亚高温处理区喷施10mmol·L-1CaCl2和0.2mmol·L-1水杨酸(SA)水溶液,以25℃喷施清水为对照,研究叶片保护酶活性(SOD、POD和CAT)和可溶性蛋白质含量的变化.结果表明:亚高温处理使叶片中SOD、CAT、POD活性降低,在处理结束时,SOD、CAT、POD活性分别比对照降低了14.82%、31.84%和26.34%.而亚高温条件下喷施CaCl2和SA可以显著降低番茄叶片MDA含量,提高SOD、POD和CAT活性,并使这些指标达到或超过对照水平.说明Ca2+和水杨酸对亚高温条件下番茄植株叶片保护酶活性具有正调控作用,这种调控作用可能对亚高温条件下番茄的光合系统起到保护作用.In order to investigate the regulation effect of Ca^2＋ and salicylic acid （SA） on the subhigh temperature resistance of tomato plants, the plants were treated with sub-high temperature （35℃ ） at day time during their 1st inflorescence flowering, and CaCl2（ 10 mmol · L^-1） and SA （0. 2 mmol · L^-1 ） were foliar sprayed to study the variations of the activities of defense enzymes （ SOD, POD, and CAT） and the content of soluble protein in tomato leaves, taking spraying clear water under 25 ℃ at day time as the control. The results showed that under the sub-high temperature stress, the SOD, CAT and POD activities in tomato leaves were decreased by 14.82%, 31.84%, and 26.34%, respectively, while spraying CaCl2（ 10 mmol ·L^-1） and SA （0. 2 mmol · L^-1） decreased the leaf MDA content and increased the leaf SOD, POD and CAT activities and soluble protein content, compared with the control, indicating that Ca^2＋ and SA had positive regulation effect on the defense enzyme activities in tomato leaves, which could protect the photosynthetic system of tomato under the sub-high temperature stress to a certain degree.

关 键 词：钙 水杨酸 番茄 亚高温 保护酶 

分 类 号：S641.2[农业科学—蔬菜学]