静脉毒瘾者84例HGV感染状况  

Prevalence of hepatitis G virus infection in 84 intravenous drug users

作　　者：李刚[1,2,3] 陈青[1,2,3] 姚集鲁 汤文辉[1,2,3] 谭德 

机构地区：[1]中山医科大学 [2]昆明医学院附属一院 [3]美国国立卫生研究院

出　　处：《中华传染病杂志》1998年第1期26-28,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金;广东省卫生厅科研基金

摘　　要：目的调查庚型肝炎病毒（ＨＧＶ）在静脉毒瘾者中的感染状况。方法采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）检测８４例静脉毒瘾者血浆标本。ＨＧＶＲＮＡ经热变性法提取后逆转录为ｃＤＮＡ，在ＨＧＶ５′非编码区（５′ＮＣＲ）设计两对引物进行巢式扩增，产物为２３８ｂｐ，并经限制性内切酶ＨｐａⅡ鉴定扩增产物来自ＨＧＶ。结果８４例中有１５例为ＨＧＶＲＮＡ阳性，阳性率为１７．９％。ＨＧＶＲＮＡ阳性病例中１１例合并丙型肝炎病毒感染（１１／１５）。结论静脉毒瘾者是ＨＧＶ感染的高危人群；不洁注射是获得ＨＧＶ感染的重要途径。Objective To investigate the epidemiology of hepatitis G virus (HGV) infection in intravenous drug users (IVDU).Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR). HGV RNA extracted by the heat denaturalization method was retro transcribed into cDNA before PCR was performed, using two pairs of primers designed from the 5′ end noncoding region of HGV. The amplified product with 238bp was identified as specific fragment of HGV by the digestion of restriction endonuclease HpaⅡ. Results The results showed that 15 of the 84(17.9%) IVDUs were positive for HGV RNA and 11 of 15 positive plasma samples were coinfected with hepatitis C virus (HGV). Conclusion IVDUs were the high risk population for HGV infection and needle sharing and no sterilizing of injectors served as important route for the transmission of HGV.

关 键 词：聚合酶链反应 庚型肝炎病毒 静脉毒瘾者 

分 类 号：R512.63[医药卫生—内科学]