乙型肝炎病毒前S1抗原检测方法及其临床意义探讨  

The Detection of Hepatitis B Virus PreSl Antigen and Its Clinical Significance

作　　者：冯福民[1] 米志宝[2] 张习坦[2] 王洪莉[3] 杨红[2] 吕宝成 

机构地区：[1]华北煤炭医学院 [2]军事医学科学院五所,100071 [3]中国人民解放军第二五四医院

出　　处：《天津医药》1998年第2期74-76,共3页Tianjin Medical Journal

摘　　要：用乙型肝炎病毒（ＨＢＶ）前Ｓ１蛋白合成肽（２１￣４７ａａ）免疫家兔制备多克隆兔抗前Ｓ１抗体，建立前Ｓ１抗原ＥＬＩＳＡ检测方法，对前Ｓ１蛋白合成肽的最低检出限为０．１μｇ／Ｌ，可测到２．５×１０＾５个／ＬＤａｎｅ颗粒。A polyclonal rabbit against PreSl antibody was prepared by linking hepatitis B virus (HBV) PreSl protein synthetic peptide (21~47aa)and keyhole limpet hemocyanin(KLH) together,and then immunizing rabbit. An ELISA kit was developed involving specific antibody for determination of PreSl antigen in our laboratory. The lower limit of the assay was about 0. ljag/L of PreSl synthetic peptide. 2. 5 X 105/L of Dane particles. Specificity and precision of the assay was very high. Using polyclonal anti-PreSl antibody could inhibit in combination PreSl synthetic peptide,gene expression PreSl protein,purified Dane particles and PreSl antigen positive serum with coated antibody, separately. The coefficient of variation,regardless pf within-group variation and between-group variation,was not over 0. 15. The positive rates of PreSl antigen in different populations were of difference , the highest in chronic activated hepatitis B,in contrast,the PreSl antigen was not detected in healthy people. During acute hepatitis B,existence of PreSl antigen and HBeAg was parallel,and disappearance of PreSl antigen was earlier than HBsAg. The presence of PreSl protein appeared to correspond wih the presence of HBV-DNA. The results indicated that the PreSl protein was a marker of HBV replication and infection,and it was more sensitive than HBsAg in reflecting HBV clearance.

关 键 词：乙型肝炎病毒 前S1抗原 ELISA 

分 类 号：R512.620.4[医药卫生—内科学] R446.61[医药卫生—临床医学]