胚胎脊髓移植兼用神经生长因子促进成年大鼠损伤脊髓功能恢复的研究  

Fetal spinal cord tissue transplantation to promote the recovery of neural function of injured spinal cord in adult rats

作　　者：沈文 王东[2] 

机构地区：[1]北京市宣武区中医院骨科,北京100050 [2]山西医科大学第二附属医院骨科,山西太原030001

出　　处：《安徽医药》2009年第3期252-256,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究胚胎脊髓(FSC)移植兼用神经生长因子(NGF)对损伤脊髓的修复作用。方法用改良Allen打击法制作脊髓损伤动物模型。将实验动物分为4组:A组:单纯脊髓损伤组;B组:脊髓损伤+NGF组;C组:脊髓损伤+移植FSC组;D组:脊髓损伤+移植FSC+NGF组。手术后应用行为学、组织形态学观察损伤脊髓功能恢复情况;应用Nissl染色方法观察脊髓神经元的大小;采用计算机图像分析技术进行定量分析。结果脊髓胚胎移植可以在宿主脊髓中存活、发育、生长、分化并形成神经连接,促进损伤脊髓功能恢复。神经生长因子可以防止成年大鼠脊髓轴突损伤引起的神经元萎缩。CBS评分、图像分析显示对脊髓损伤功能恢复的作用,B、C、D组好于A组,B、C组间无显著差异,D组效果最好。FSC移植与运用外源性NGF对于促进成鼠损伤脊髓功能恢复具有协同效应。结论胚胎脊髓移植兼用神经生长因子能维持神经元的细胞形态,对成年大鼠损伤脊髓功能恢复有促进作用。Aim To study the recovery of neural function of injured spinal cord by fetal spinal cord tissue transplantation and nerve growth factor. Methods Animal model of SCI was made by modified Allen＇s method. The rats were divided into the following 4 groups： group A with hemisection of the cord only ; group B with cord hemisection and NGF ; group C with cord hemisection and fetal cord grafting;group D with cord hemisetion, fetal cord grafting and NGF. After operation, combined behavioral scores were examined. Pathological changes of spinal cord tissue were observed with light microscopy. The slides with Nissl staining were used to measure the size of the neurons. The positive cells were quantitatively analyzed with a computer image analysis system. Results The transplantation of FSCS into the spinal cord cavity of adult rats formed following spinal cord injury can survive, grow, differentiate and form neurons connection. NGF may salvage the injured neurons. The recovery of neural function of injured spinal cord was better in group D, C, B than A as evidenced by CBS scores and morphological observation. The neurofunctional recovery was the best in group D. There may be a synergistic effect between FSC and NGF. Conclusion The combination of the transplantation of fetal spinal cord and nerve growth factor ean promote the structural and functional recovery of the injured neurons.

关 键 词：脊髓损伤 移植 胚胎脊髓 神经生长因子 修复 

分 类 号：R651.2[医药卫生—外科学]